单环刺螠多肽抗肿瘤及对小鼠免疫功能的调节作用

目的：进行单环刺螠多肽体外抑制人肝癌细胞（SMMC-7721）生长的实验，研究其对S180肉瘤的抑瘤作用及对小鼠机体免疫力的影响。方法：通过MTT法测定不同浓度单环刺螠多肽对人肝癌细胞和正常肝细胞的影响;采用灌胃给药的方式，观察该多肽对S180荷瘤小鼠瘤重的影响；采用灌胃的方法研究其对正常昆明种小鼠免疫系统的影响。结果：单环刺螠多肽对人肝癌细胞有抑制作用，且存在剂量依赖性，对正常人肝细胞无明显毒副作用；体内实验发现多肽对小鼠S180肉瘤具有一定的抑制作用，并能剂量依赖性地提高小鼠脾指数和胸腺指数及其他免疫指标。结论：单环剌螠多肽在体外抑制人肝癌细胞生长，并可以增强小鼠免疫力，抑制小鼠S180肉瘤的生长。

Antitumor Effect and Immunomodulation of Polypeptides from Urechis unicinctus in Mice

AIM： To study the growth inhibitory effects of polypeptides from Urechis unicinctus on human hepatoma SMMC-7721 cells. The antitumor activity and the effects on immunity were also investigated. METHODS： The effects of the polypeptides on normal human liver cells and human hepatoma SMMC-7721 cells were examined with MTT assay. The polypeptides was orally administered to S180 KM mice, the inhibition rates was calculated. The same way to KM mice, the effects on immunity was also calculated. RESULTS： Using the method of MTT, it was found that the polypeptides inhibited the growth of tumor cell, and had no effect to normal liver cells. The polypeptides were also promoted the activity of mouse immunity system. CONCLUSION： With the dosage of polypeptides increasing, the inhibitory effects of the polypeptides on hepatoma was strengthened, the polypeptides from Urechis unicinctus had the ability to up-regulate the immunity in vivo.