HPLC法测定壮骨关节胶囊中补骨脂素、异补骨脂素的含量

目的:建立壮骨关节胶囊中补骨脂素、异补骨脂素含量HPLC方法。方法:色谱柱为Alltima C₁₈（250 mm×4.6 mm,5μm）,流动相为甲醇-水（50:50）,流速为1.0 ml·min^-1,检测波长为246 nm。结果:补骨脂素和异补骨脂素分别在1.92～96.24μg·ml^-1（r=1.000 0）和20.46～102.32μg·ml^-1（r=1.000 0）范围内线性关系良好,回收率分别为104.1%（RSD=0.8%）和99.9%（RSD=1.6%）。结论:本法简便、准确、稳定且无干扰,可用于壮骨关节胶囊的质量控制。

Determination of Psoralen and Isopsoralen in Zhuanggu Guanjie Capsules by HPLC

Objective： To establish a method for the determination of psoralen and isopsoralen in Zhuanggu Guanjie capsules by HPLC. Method： An Alhima C18 （250 mm × 4. 6 mm,5 μm） column was used, the mobile phase was methanol-water（ 50： 50, v/v）, the flow rate was 1.0 ml·min ^- 1 and the detection wavelength was 246 nm. Result ： The linear range of psoralen and isopsoralen were 1.92- 96. 24 μg· ml^ - 1 （ r = 1. 000 0 ） and 20. 46-102. 32 μg· ml ^- 1 （ r = 1. 000 0 ） with the average recovery of 104. 1% （ RSD = 0. 8 % ） and 99. 9% （RSD = 1.6% ）, respectively. Conclusion： This method is simple, accurate and stable. It can be used for the quality control of Zhuanggu Guanjie capsules.