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| 苦豆草药材质量标准的研究 | |
| 引用本文： | 冷晓红,马玲,王俊卿,王英华.苦豆草药材质量标准的研究[J].中国药品标准,2011,12(4):285-288. |
| 作者姓名： | 冷晓红 马玲 王俊卿 王英华 |
| 作者单位： | 1. 宁夏职业技术学院,银川,750002 2. 宁夏药品检验所,银川,750001 3. 宁夏回族自治区中医研究院,银川,750006 |
| 基金项目： | 宁夏"十一五"科技攻关项目 |
| 摘 要： | 目的:建立苦豆草药材的质量标准。方法:采用TLC法对苦豆草药材进行定性鉴别;用HPLC法测定氧化苦参碱和槐定碱的含量。结果:薄层色谱图显示,氧化苦参碱和槐定碱与其他生物碱分离效果良好,可以用于苦豆草药材的鉴别;高效液相色谱中,氧化苦参碱和槐定碱分别在25.104～125.52μg和22.824～114.12μg范围内线性关系良好,平均加样回收率(n=9)分别为96.2%和95.9%。结论:所建立的方法可准确地进行定性、定量分析,可用于苦豆草药材的质量控制。 |
| 关 键 词： | 苦豆草 氧化苦参碱 槐定碱 薄层色谱 高效液相色谱 |
Study on quality standard of Herba Sophora Alopecuroides |
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| Authors: | Leng Xiao-hong Ma Ling Wang Jun-qing and Wang Ying-hua |
| Institution: | Leng Xiao-hong1,Ma Ling2,Wang Jun-qing3,Wang Ying-hua2(1.Ningxia Vocational and Technical College,Yinchuan 750002,2.Ningxia Institute for Drug Control,Yinchuan 750001,3.Ningxia Acdemy of Traditional Chinese Medicine,Yinchuan 750004) |
| Abstract: | Objective：To establish the quality standard of Herba Sophora Alopecuroides . Methods： The TLC and HPLC methods were used for qualitative identification and quantitative determination of oxymatrine and Sophridine. Cis column （4. 6 mm ×250 mm,5 μm） was used. The mobile phase consisted of 0. 05 mol · L^-1K2 H2 PO4 solution-acetonitrile （90： 10） （2.0 mL · L^-1 triethylamine ） . The UV detective wavelength was 205 nm. Results： The identified characteristics of Oxymatrine and Sophridine were distinct and Her- ba Sophora Alopecuroides could be identified by TLC. In the system of HPLC, the linear range for the content of Oxymatrine and Sophridine were as the of 25. 104 - 125.52 μg （r =0. 9991） and 22. 824 ~ 114. 12 μg （r =0. 999 7）. Conclusion： The method can be used for the quality control of Herba Sophora Alopecuroides. |
| Keywords: | Herba Sophora Alopecuroides oxymatrine Sophridine TLC HPLC |
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