N-三甲基壳聚糖对尼莫地平凝胶透皮吸收促进作用的体外研究

目的：考察N-三甲基壳聚糖对尼莫地平凝胶透皮吸收促进作用。方法：以季胺化程度为60％的，N-三甲基壳聚糖（TMC60）为透皮吸收促进剂，以泊洛沙姆407为凝胶基质，制备尼莫地平凝胶（TMC60组）。用Franz扩散池进行体外透皮研究，并与含同浓度的氮酮（氮酮组）、油酸（油酸组）的尼莫地平凝胶及不含促进剂的尼莫地平凝胶（空白组）的效果进行比较。结果：空白组、氮酮组、TMC60组及油酸组的稳态透皮速率（J，μg·cm^－2·h^-1）分别为6．59，13．22，16．39和16．77。与空白组相比，TMC60尼莫地平凝胶有显著的透皮吸收促进作用（P〈0．05），且作用强度明显优于氮酮（P〈0．05），与油酸相似（P〉0．05）。结论：TMC60可较好地促进尼莫地平凝胶的体外透皮扩散。

Study on Transdermal Penetration Enhancement of N-trimethyl Chitosan on Nimodipine Gel in vitro

Objective： To study the enhancement of N-trimethyl chitosan on transdermal penetration of nimodipine gel in vitro. Method： N-trimethyl chitosan with quaternization of 60% （TMC60） as the enhancer, poloxamer 407 as the gel base, the nimodipine gel was prepared. The enhancement of TMC60,Azone and oleic acid with the same concentration （3%） was studied and compared u- sing Franz diffusion cells in vitro. Result： Stable penetration rate （J, μg·cm^-2·h^-1 ） of the nimodipine gel without any enhancer and with Azone, TMC60 and oleic acid as the enhancer was 6. 59,13.22,16. 39 and 16. 77, respectively. Compared with the blank group, TMC60 had significant enhancement （ P 〈 0. 05 ）, and the effect was better than Azone （ P 〈 0. 05 ） and similar with oleic acid （ P 〉 0. 05 ）. Conclusion： TMC60 can enhance transdermal penetration of nimodipine gel in vitro.