流动相对灰树花多糖相对分子质量测定影响的考察

目的:考察流动相对高效凝胶渗透色谱法（HPGPC）测定灰树花多糖（GFP）相对分子质量（Mr）的影响因素。方法:采用HPGPC法分别以不同的流动相、盐浓度、pH测定GFP的Mr,并对测定结果进行分析。结果:分别以纯水、0.02%NaN₃、0.1mol·L^-1NaNO₃和0.1 mol·L^-1NaCl溶液为流动相进行测定,重均相对分子质量（WAMr）分别为822410、177520、29112、25066道尔顿,不同的流动相GFP的Mr有明显不同,以0.02%NaN₃较为理想;在低盐浓度(0～0.025mol·L^-1)下,Mr变化明显,在0.1～0.2mol·L^-1硫酸钠溶液中趋于平稳;在pH 3～6时,Mr随pH增加而急剧增加,在pH 6～9范围内变化不明显。结论:不同流动相、盐浓度、pH对GFP的Mr测定结果有明显影响。

Influence of Mobile Phases on Molecular Weight Determination of Grifola Frondosa Polysaccharides

Objective：To investigate the influence of mobile phases on relative molecular weight（Mr）determination of Grifola Frondosa Polyaccharide（GFP）by high performance gel permeation chromatography（HPGPC）.Method：The Mr of GFP was determined by HPGPC using different mobile phases,concentrations of NaCl and pH value.Result：The Mr of GFP was 822 410,177 520, 29 112 and 25 066 Dalton determined by HPGPC using pure water,0.02%NaN_3 solution,0.1 mol·L^-1NaNO3 solution and 0.1 mol·L^-1NaCl solution as the mobile phase,respectively.The Mr of GFP was sharply dropped using 0-0.025 mol·L^-1Na2SO4 as the mobile phase and then tended to be stable using 0.1-0.2 mol？L^-1Na2SO4.The Mr increased quickly with pH of 3 to 6,and then maintained stable with 6 to 9,while slightly increased at 10.Conclusion：Different mobile phases,concentrations of Na2SO4 and pH values have great influence on the Mr determination of GFP.