| HPLC法测定消炎片中蒙花苷的含量 |
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| 引用本文： | 陈立柱,刘辉,潘鹏飞. HPLC法测定消炎片中蒙花苷的含量[J]. 中国药师, 2011, 14(11): 1601-1603 |
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| 作者姓名： | 陈立柱 刘辉 潘鹏飞 |
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| 作者单位： | 1. 鸡西市食品药品检验检测中心,黑龙江鸡西,158100
2. 鸡西市传染病医院 |
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| 摘 要： | 目的:建立反相高效液相色谱法测定消炎片中蒙花苷的含量。方法:采用AgiLent EcLipse XDB-C18（250mm×4.6mm,5μm）色谱柱,以甲醇-乙腈-0.04%磷酸溶液为流动相,梯度洗脱;检测波长为334nm;流速1.0ml·min-1;进样量10μl;柱温35℃。结果:蒙花苷的线性范围为0.0396～0.7927μg,r=1.0000（n=7）;提取回收率为98.76%,RSD为0.5%（n=6）。结论:本法操作简便、快速、结果准确,可用于消炎片的质量控制。
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| 关 键 词： | 消炎片 含量测定 蒙花苷 高效液相色谱法 |
Determination of Buddleoside in Xiaoyan Tablets by HPLC |
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| Chen Lizhu,Liu Hui,Pan Pengfei. Determination of Buddleoside in Xiaoyan Tablets by HPLC[J]. China Pharmacist, 2011, 14(11): 1601-1603 |
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| Authors: | Chen Lizhu Liu Hui Pan Pengfei |
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| Affiliation: | 1. Jixi Food and Drug Testing Center, Heilongjiang Jixi 158100, China;2. Jixi Infectious Disease Hospital) |
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| Abstract: | Objective ： To establish an RP-HPLC method for the determination of buddleoside in Xiaoyan tablets. Method ： An Ag- ilent Eclipse XDB-C18 （250 mm ×4. 6 mm,5μm） column was used. The mobile phase was methanol-acetonitrile-0. 04% H3PO4 with gradient elution and the detection wavelength was 334 nm. The flow rate was 1.0 ml.min-1 ,the injection volume was 10 μl and the col- umn temperature was 35 ℃. Result： The linear range of buddleoside was from 0. 039 6-0. 792 7 μg （r = 1. 000 0,n =7）. The average recovery of buddleoside was 98. 76% （ RSD = 0. 5% , n = 6）. Conclusion： The method is convenient, quick and accurate, and can be used in the quality control of Xiaoyan tablets. |
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| Keywords: | Xiaoyan tablets Determination Buddleoside HPLC |
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