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| 中国被毛孢菌丝体提取物抗肾损伤作用体内外实验研究 | |
| 引用本文： | 沈龙海,安泳潼,杨庆尧,杨晓彤,沈旭,丁宏. 中国被毛孢菌丝体提取物抗肾损伤作用体内外实验研究[J]. 中国药理学通报, 2011, 27(11): 1537-1540. DOI: 10.3969/j.issn.1001-1978.2011.11.015 |
| 作者姓名： | 沈龙海 安泳潼 杨庆尧 杨晓彤 沈旭 丁宏 |
| 作者单位： | 1. 上海医药工业研究院创新药物与制药工艺国家重点实验室,上海,200437 2. 上海芝草生物技术有限公司,上海,200233 3. 中国科学院上海药物研究所,上海,201203 |
| 基金项目： | 国家"重大新药创制"科技重大专项资助项目 |
| 摘 要： | 目的探讨中国被毛孢菌丝体提取物(HSW)抗肾损伤的药理作用。方法采用体外培养的人肾小管上皮细胞株(HK2)模型,观察HSW对马兜铃酸(AA)造成的HK2细胞损伤的逆转作用,及对于肾衰竭相关功能指标的影响。并在庆大霉素致大鼠急性肾衰竭(ARF)模型上验证HSW的作用。结果 HSW可以逆转AA对HK2细胞所造成的损伤,有效地抑制AA引起的HK2细胞TGFβ1、PAI-1 mRNA表达的上调,并可保护庆大霉素造成的大鼠急性肾损伤。结论 HSW对肾损伤的体内外模型有较好的药理作用。 |
| 关 键 词： | 中国被毛孢菌丝体提取物 体内外作用 肾小管上皮细胞株 细胞损伤 大鼠急性肾衰竭 TGFβ1 PAI-1 |
In vitro and in vivo study of Hirsutella sinensis extract on kidney injury |
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| SHEN Long-hai,AN Yong-tong,YANG Qing-yao,YANG Xiao-tong,SHEN Xu,DING Hong. In vitro and in vivo study of Hirsutella sinensis extract on kidney injury[J]. Chinese Pharmacological Bulletin, 2011, 27(11): 1537-1540. DOI: 10.3969/j.issn.1001-1978.2011.11.015 | |
| Authors: | SHEN Long-hai AN Yong-tong YANG Qing-yao YANG Xiao-tong SHEN Xu DING Hong |
| Affiliation: | SHEN Long-hai1,AN Yong-tong1,YANG Qing-yao2,YANG Xiao-tong2,SHEN Xu3,DING Hong3(1.State Key Lab of Innovative Medicines and Pharmaceutical Technology,Shanghai Institute of Pharmaceutical Industry,Shanghai 200437,China,2.Shanghai Zhicao Biotechnology Co.Ltd,Shanghai 200233,3.Chinese Academy of Sciences,Shanghai Institute of Pharmaceutical Research,Shanghai 201203,China) |
| Abstract: | Aim To investigate the effect of Hirsutella sinensis extract(HSW) on kidney injury.Methods The inversion effect of HSW on the renal tubular epithelial cell(HK2) injury caused by aristolochic acid(AA) was observed,its influence on functional parameters of renal failure was evaluated and the effect of HSW on acute renal failure(ARF) of rats induced by gentamicin was validated.Results HSW deteriorated HK2 cell damage caused by AA,inhibited the upregulation of the expression of TGFβ1 and PAI-1 mRNA,and protected rats from ARF caused by gentamicin.Conclusion HSW had a remarkable action in vitro and in vivo on model of kidney injury. |
| Keywords: | Hirsutella sinensis extract in vitro and in vivo renal tubular epithelial cell cell injury acute renal failure TGFβ1 PAI-1 |
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