四氯化碳诱导大鼠肝纤维化进展期和恢复期模型的建立

目的确定肝纤维化进展期和恢复期。方法采用50%四氯化碳(carbon tetrachloride,CCl4)皮下注射建立大鼠肝纤维化和自然恢复模型。检测血清中丙氨酸氨基转氨酶(alanine aminotransferase,ALT)、天冬氨酸氨基转移酶(as-partate aminotransferase,AST)、透明质酸(hyaluronic acid,HA)、层粘连蛋白(laminin,LN)、Ⅳ-C型胶原(collagenⅣ,Ⅳ-C)和Ⅲ型前胶原(procollagenⅢ,PCⅢ)含量及肝组织羟脯氨酸(hydroxyproline,Hyp)水平;HE染色和Masson胶原染色观察肝脏病理组织学改变。结果 CCl4注射8周,大鼠肝组织己出现慢性肝炎的病理变化,注射12～16周出现了典型的从肝纤维化—肝硬化的病理改变,同时大鼠血清中ALT、AST、HA、LN、Ⅳ-C和PCⅢ含量及肝组织Hyp水平逐渐上升。而随着恢复期的延长,血清中ALT、AST、HA、LN、Ⅳ-C和PCⅢ含量及肝组织Hyp水平逐渐下降。结论成功建立CCl4诱导的大鼠自愈性肝纤维化模型,并确定进展期和恢复期。

Establishment of the progressive and recovery fibrosis model in rats induced by carbon tetrachloride

Aim To establish the model of progression and recovery of hepatic fibrosis in rats.Methods Progressive and recovery fibrosis model was induced by subcutaneous injection of CCl4.The levels of HA,LN,Ⅳ-C and PCⅢ in serum and Hyp in liver were observed.Results Histological analysis of the livers harvested after treatment CCl48,12,16 weeks showed pathologic change of chronic hepatics,hepatic fibrosis and hepatic cirrhosis,respectively.Meanwhile,the levels of HA,LN,Ⅳ-C and PCⅢ in serum and the Hyp in liver were elevated gradually.Whereas,with recovery from fibrosis,the levels of HA,LN,Ⅳ-C and PCⅢ in serum and the Hyp in liver were markedly diminished and pathologic change of hepatic tissue was improved.Conclusion The model of progression and recovery of hepatic fibrosis is successfully established.