Analysis of human T-cell receptor alpha-chain cDNAs isolated from peripheral blood mononuclear cells. |
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Authors: | M Yassai M Bull J Gorski |
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Affiliation: | Immunogenetics Research Section, Blood Center of Southeastern Wisconsin, Milwaukee 53233. |
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Abstract: | T-cell receptor alpha-chain cDNA were generated from unstimulated peripheral blood mononuclear cells of a DR2,3,52a individual using a modified anchor PCR method. Fifty-six cDNA clones were identified representing 47 distinct T-cell receptor clonotypes and 26 VA loci. This analysis identified a new VA gene family VA30, and a new member of the VA6 gene family. |
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