首页 | 本学科首页   官方微博 | 高级检索  
     

猪急性肾衰竭并多器官功能障碍模型建立及病理观察
引用本文:王恒进,张苗,孙(王争). 猪急性肾衰竭并多器官功能障碍模型建立及病理观察[J]. 中国中西医结合肾病杂志, 2008, 9(6): 502-506
作者姓名:王恒进  张苗  孙(王争)
作者单位:南京大学医学院附属鼓楼医院,南京210008
基金项目:国家自然科学基金 , 南京大学附属医院青年创新基金
摘    要:目的:建立一个重症感染后多器官功能障碍综合征(MODS)并急性肾衰竭(ARF)复合的猪模型。方法:16只健康杂交家猪随机分成模型组和假手术对照组。模型组行盲肠结扎和穿孔(CLP),加作肾动脉夹闭(CRA)制作腹腔感染致MODS并ARF模型,对照组行盲肠和双肾探查术。观察生命体征及主要器官功能(MODS相关指标)变化。结果:模型组术后24~72h出现器官功能障碍:(1)ARF:血清肌酐(Scr)、尿素氮(BUN)分别从(92.70±11.05)μmol/L、(4.16±1.02)mmol/L升至(986.37±132.61)μmol/L、(48.13±2.68)mmol/L。(2)肝功能:血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)均超过对照值的2倍;(3)凝血功能:血小板数〈80%对照值,且皮肤可见弥漫或散在的出血点;(4)心功能:平均动脉压(MAP)持续≤70%对照值,且呈进行性下降;心率≥130次/min;(5)肺功能:动脉血二氧化碳分压(PaO2)下降,低于69.75mmHg;呼吸频率增快,均值超过照值的2倍;(6)胃肠功能障碍;(7)肾、肝、脾、肺及小肠等器官组织病理上均有不同程度的实质性改变。模型组动物6d内全部死亡,平均存活时间(69.81±22.59)h;对照组全部存活,且器官功能无明显变化。结论:采用CLP+CRA方法,可以成功地复制出MODS并ARF复合模型,并可见最终均发展为多器官衰竭。

关 键 词:多器官功能障碍综合征  急性肾衰竭  猪模型  病理学

Establishment and Pathological Observation of A Pig Model with Acute Renal Failure and Multiple Organ Dysfunction Syndromes
WANG Hengjin,ZHANG Miao,SUN Cheng. Establishment and Pathological Observation of A Pig Model with Acute Renal Failure and Multiple Organ Dysfunction Syndromes[J]. Chinese Journal of Integrated Traditional and Western Nephrology, 2008, 9(6): 502-506
Authors:WANG Hengjin  ZHANG Miao  SUN Cheng
Affiliation:WANG Hengjin,ZHANG Miao,SUN Cheng( Department of Nephrology,the Affiliated Drum Tower Hospital of Nanjing University Medical School,Nanjing,210008)
Abstract:Objective:To establish a composite pig model with multiple organ dysfunction syndrome(MODS) and acute renal failure(ARF).Methods:Sixteen pigs were randomly divided into two groups:group E received cecal ligation and puncture(CLP) plus clamping of the renal artery(CRA) and group C received sham operation.Vital signs and the functions of the main organs were observed.Results:Seventy-two hours after operation,animals in the model group showed ARF and MODS-like changes:(1)Gastrointestinal dysfunction;(2)The enhanced indexes of renal and liver function,including serum Cr,BUN,ALT and AST;(3)Pulmonary dysfunction,such as PaCO2 from(38.04±2.98)mmHg to(52.14±4.62)mmHg(P〈0.01);(4)Cardiac dysfunction,such as the reduction of MAP and the increase of heart rate;(5)Dysfunction of blood coagulation,such as the decrease of blood platelet;(6)The survival time in group E was(69.81±22.59) hours.(7)Severe pathological lesions were observed in the lung,liver,heart,kidney and gut,etc.Conclusion:In the course of MODS and ARF caused by intra-abdominal infection and clamping of the renal artery,the main organs deteriorated progressively and it finally became multiple organ failure.
Keywords:Multiple organ dysfunction syndrome Acute renal failure Pig model Pathology
本文献已被 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号