组织工程软骨修复兔膝关节软骨大面积深层缺损的实验研究

目的　将培养的软骨细胞埋在胶原凝胶中，观察其对家兔漆关节软骨大面积深层缺损的修复作用。方法体外培养软骨细胞，然后与胶原以一定比例混合培养，植入免膝关节股骨负重面的缺损（５ｍｍ ×４ｍｍ）部位，观察 ２、４、８．１２周软骨缺损的修复情况，并进行大体和组织学观察。结果移植后第 ８周，所修复组织的边缘变得模糊，与周围宿主整合得很好，缺损边缘不清晰。组织学观察，缺损处表面光滑，边缘与宿主完全整合，缺损处呈典型的关节软骨样组织，并且底部有新形成的软骨及骨小梁生成。对照组（未移植组）组织学观察缺损部位主要为纤维组织所填充，未见有由新生软骨细胞形成的软骨组织。结论埋在胶原凝胶中软骨细胞对家兔膝关节软骨深层缺损的修复作用。

Tissue engineering cartilage repair of large, full-thickness defects of articular cartilage

Repair of large, full-thickness defects of rabbit articular cartilage with allograft chondrocytes embedded in collagen gel. At eight weeks alter the operation, the repairative tissue became opaque and well incorporated. The margins of the defects were nuclear and it was very difficult to discern the boundary between the host and the repairative tissue. Histological observed the surface of the defect was smooth, the margins were nuclear. Reformed cartilage and cancellous at the base of the defects.