基于CRISPR/Cas9研究RFX6对斑马鱼胰腺发育的影响

目的 构建RFX6基因敲除斑马鱼动物模型及初步探究RFX6对斑马鱼胰腺发育的影响。方法 应用CRISPR/Cas9技术,设计针对靶点的gRNA,诱导靶点突变,经测序鉴定得到突变体。结果 成功构建了RFX6基因敲除斑马鱼动物模型,初步研究发现RFX6纯合突变体生长发育迟滞、体型瘦小、呈现糖尿病消瘦体型并且不能产生后代。结论 RFX6在斑马鱼胰腺的发育中占据重要地位,是控制斑马鱼胰腺发育的关键因子。

Effect of RFX6 on pancreas development in zebrafish using CRISPR/Cas9

Objective To construct the RFX6 knockout zebrafish animal model and preliminarily study the effects of RFX6 on the pancreatic development in zebrafish. Methods CRISPR/Cas9 technology was used to design gRNA against the target and induce mutation, thus the mutants can be obtained by sequencing. Results The RFX6 knockout zebrafish animal model was successfully constructed. Preliminary studies showed that RFX6 homozygous mutants suffered from growth retardation, lean and thin body with diabetes, and could not produce offsprings. Conclusion RFX6 plays an important role and is the key factor in the pancreatic development of zebrafish.