一氧化氮在顺铂致大鼠肾损害过程中的作用

目的 探讨一氧化氮在顺铂肾毒性氧化应激机制中的作用。方法 采用少量多次给大鼠腹腔注射顺铂（ＣＰ）及经口给予水飞蓟素（ＳＢ）预处理后给予ＣＰ模型，观察血尿素氮（ＢＵＮ）含量、一氧化氮合酶（ＮＯＳ）活性、丙二醛（ＭＤＡ）形成、超氧化物歧化酶（ＳＯＤ）活性等指标的变化。结果 ＣＰ可诱导ＮＯＳ活性增高，使ＮＯ生成量增多；ＢＵＮ含量与ＭＤＡ含量及ＳＯＤ活性的变化并不完全一致，而与ＮＯ含量的时相变化活性增高，

Role of nitric oxide in cisplatin-induced kidney damage in rats

Objective To explore the role of nitric oxide in oxidative stress mechanisms in cisplatin induced kidney damage in rats. Methods Fifty four rats were randomly divided into three groups of 18 rats each.The three groups were given normal saline,CP(1 mg/kg/day) intraperitoneally or SB(24 mg/kg/day) orally,1 hour prior to CP,respectively.Having been treated daily for 7 days,six animals of each group were killed at day 2,day 5 or day 10 after the last administration.The indicators included the level of blood urea nitrogen (BUN),the formation of malondialdehyde (MDA),Nitric oxide (NO) content,the activity of superoxide dismutase (SOD),the histochemical change of nitric oxide synthase (NOS),etc. Results The results showed that the increase of the productions of NO by increasing the activities of NOS was more related to the nephrotoxicity induced by CP than the decrease of the activities of SOD and the increase of MDA contents related.The pretreatment of SB could ameliorate the nephrotoxicity induced by CP,and the possible mechanism was related to decrease NO production and to increase activity of SOD. Conclusion NO maybe play an important role in nephrotoxicity induced by CP.