抗氰戊菊酯淡色库蚊的抗性谱以及增效剂的作用

在室内用氰戊菊酯对采集于野外的淡色库蚊进行了选育,获得一个抗性高达6088倍的高抗性品系(RPy),并测定了该品系对常用杀虫剂的交互抗性以及增效剂作用。结果表明,RPy品系对7种拟除虫菊酯的交互抗性范围为6(胺菊酯)～140倍以上(反式-氯菊酯);对P,P′-DDT的交互抗性>388倍;对狄氏剂、乐果、敌百虫和残杀威的交互抗性为1～2倍;对丙体六六六、杀螟硫磷,甲基对硫磷,马拉硫磷、双硫磷和甲萘威的敏感度高于敏感品系。应用各种专一性抑制剂pb、TPP和DMC进行了活体增效作用的研究。结果表明,RPy品系对P,P′DDT的抗性主要与DDT脱氯化氢酶(DDTase)和多功能氧化酶(MFO)有关;对氰戊菊酯的抗性主要与MFO和酯酶有关。

Cross-resistance Spectrum and Effects of Synergists in the Fenvalerate-resistant Mosquito,Cutex pipiens pallens(coq.)

A high level of 6088-fold population (RPy strain) of fenvalerate resistant Culex pipiens pallens(Coq.)collected from Nanshi district in Shanghai was successfully developed in laboratory by treating the 4th instar larvae with fenvalerate. The RPy strain showed high cross-resistant level for P, P'-DDT(>388X), for other Pyrethroids 6(Tetramethrin) to>140X (tran-permethrin)]. for Dieldrin, Dimethoate, Trichlorfon and Propoxur (1-2X), and for Fenitrothion, Methylparathion, Malathion, Temephos and Carbaryl (more susceptible than S strain). The in vivo studies of synergism by different special inhibitors such as Pb, TPP and DMC indicated that mechanisms of resistance in the RPy strain might be associated with DDTase and mixed-function oxidase (MFO) for DDT-resistance, and MFO and Esterase for fenvalerateresistance.