热休克蛋白90抑制杀伤效应剂对PC-3M细胞的研究

目的：观察热休克蛋白90（HSP90）抑制剂对前列腺癌PC3M细胞增殖活性、细胞周期和凋亡的影响，初步探讨其作用机制。方法：采用四甲基偶氮唑盐（MTT）比色法检测不同浓度的HSP90抑制剂对PC3M细胞增殖的影响；采用细胞免疫化学测定HSP90抑制剂对PC3M细胞HER-2表达的影响；采用流式细胞分析术（FCM）检测细胞凋亡和细胞周期。结果：高浓度（500nmol／L）HSP90抑制剂可杀伤PC3M细胞，低浓度（10、50、125、250nmol／L）条件下则主要发挥生长抑制作用。HSP90抑制剂可使PC3M细胞HER-2表达水平降低，并在500nmol／L浓度时诱导细胞凋亡。结论：HSP90抑制剂较为明显地抑制PC-3M细胞生长，并在高浓度时杀伤癌细胞；其作用机制可能与下调HER-2的表达有关。

Influence of HSP90-inhibitor on the proliferation of prostate carcinoma cells PC-3M

Objective:To examine the effects of HSP90-inhibitor on the growth, cell cycle and apoptosis of PC-3M cell line, and to explore the possible mechanisms.Methods:The effects of diverse concentrations HSP90-inhibitor on the proliferation of PC-3M cell line were assayed by rypan blue exclusion and tetrazolium (MTT). The expression of HER-2 in PC-3M was assayed by immunohistochemical SP method. The effects of HSP90-inhibitor on the cell cycle distribution and apoptosis induction of PC-3M were evaluated with flowcytometry (FCM).Results:A higher dosage of HSP90-inhibitor (500 nmol/L) had a cytotoxic effect on PC-3M cells, while lower dosage from 10nmol/L to 250nMol/L produced a predominant inhibiting effect. FCM analysis manifested that HSP90-inhibitor high dosage of 500 nmol/Lcould induced apoptosis, and at the other concentrations, G1 arrest.Conclusions:HSP90-inhibitor could suppress the proliferation of PC-3M cell line and induced apoptosis at high dosage. This effect might result from reducing the expression of HER-2 in PC-3M cell line.