犬肝纤维化MR弥散加权成像及VEGF表达与病理对照研究

目的：分析磁共振弥散加权成像参数ADC值、eADC值及VEGF表达水平在中华田园犬肝纤维化进程中的变化规律；并与肝脏组织常规病理结构改变进行对照分析，探讨肝纤维化的诊断方法。方法：采用腹腔注射50%四氯化碳（CCL4）油溶液配合高脂饮食，建立犬肝纤维化的动物模型，定期对其行MR常规扫描、DWI及肝穿刺，对肝脏组织进行常规病理学染色及VEGF免疫组织化学染色，根据病理分期分组，分析各组ADC值、eADC值及VEGF表达水平的变化规律及与肝纤维化的相关性。结果：各期肝纤维化模型：S0 （n=12）、S1 （n=9）、S2 （n=9）、S3 （n=10）、S4 （n=10）；纤维化程度加重，ADC值减低、eADC值增高，差异均有统计学意义（P〈0.05）。ADC值及eADC值与肝纤维化程度有很好的相关性；随肝纤维化程度加重，VEGF表达水平明显增高。结论：四氯化碳油溶液腹腔注射配合高脂饮食可以成功建立不同分期犬肝纤维化模型。ADC值及eADC值能够定量分析不同程度的肝纤维化，是诊断不同程度纤维化较有意义的评价指标。VEGF可能在慢性肝病所致肝纤维化过程中起重要作用。

The study of both MR diffusion-weighted imaging and expression of VEGF compared with pathology in dog model of liver fibrosis

Objective： To evaluate the change of diffusion-weighted MR imaging markers and expression of VEGF in dog model of liver fibrosis, and compared with histopathological features to investigate the diagnostic methods for liver fibrosis. Materials and Methods： 50% of the carbon tetrachloride （CCL4） oil solution were injected in peritoneal cavity in dogs, and were received the high fat diet. The dogs were underwent MR examination and liver biopsy every 4 weeks. The liver tissue were underwent pathology routine HE staining, Masson staining and immunohistochemistry VEGF staining, divided into groups by the results of pathological stage, the level of VEGF expression, the corresponding ADC values and eADC values were compared among different stages of liver fibrosis, analyzing the correlations among them. Results： Grouping according to different stage of fibrosis, SO （n=12）, S1 （n=9）, $2 （n=9）, $3 （n=10）, $4 （n=10）. ADC values decreasing and eADC values increasing as the stage of fibrosis progressed （P〈0.05）, There was a good relationship between ADC （eADC） and stage of fibrosis. The expression of VEGF increased with increasing degrees of liver fibrosis. Conclusions： An intraperitoneal injection of carbon tetrachloride in oil solution with the high fat diet in dogs can be established different stages of liver fibrosis, The value of ADC （eADC） can quantitatly analysis deferent degree hepatic fibrosis, they are more meaningful evaluation indicators to evaluate the hepatic fibrosis. VEGF may play an important role in the process of liver fibrosis in chronic liver disease.