姜黄素诱导HO-1表达对大鼠动脉粥样硬化的影响

目的探讨姜黄素能否诱导大鼠主动脉血红蛋白氧合酶-1(HO-1)高表达,以及诱导HO-1高表达能否有效发挥内源性抗动脉粥样硬化(AS)作用。方法以健康雄性Wistar大鼠38只,随机分为正常对照组(n=8只)、模型组(n=14只)、姜黄素组(n=8只)及抑制组(n=8只),模型组、姜黄素组、抑制组同法复制AS模型,姜黄素组加用姜黄素,抑制组加用姜黄素及锌原卟啉Ⅸ。于6、10及14周末分别随机处死模型组大鼠2只以了解AS形成程度。第14周末处死所有大鼠取降主动脉观察病理学变化,同时行主动脉内HO-1表达、分布情况及活力测定。结果 (1)姜黄素组HO-1表达及活力均显著高于模型组(P<0.05),AS程度明显轻于模型组。(2)抑制组HO-1表达及活力均明显低于姜黄素组(P<0.05),AS程度较姜黄素组加重。结论姜黄素可显著诱导HO-1表达并增加其活力进而发挥抗AS作用。

Effect of curcumin by inducing heme oxygenase-1 expression on atherosclerosis in rats

Objective To explore whether curcumin(CMN)could induce the expression of heme oxygenase-1(HO-1) in rat’s aortic artery and the induced high expression plays an anti-atherosclerosis(AS) role.Methods 38 male Wistar rats were randomly divided into 4 groups as control group(group A,n=8),model group(group B,n=14),treatment group(group C,n=8) and suppression group(group D,n=8).Rate model with AS was established by same method in group B、C and D,group C were treated with CMN and group D treated both CMN and ZnPP Ⅸ at the same time.2 rats from group B were executed at the end of 6,10 and 14 weeks respectively to detect the pathological changes and the AS degree.All of the rates were executed at the end of 14 weeks’ treatment and the descending aorta tissue was observed by light microscopy,furthermore,detected the expression and distribution and its activity about HO-1 protein in the aortic artery.Results(1)The expression and activity about HO-1 in group C were increased significantly than group B(P<0.05),the degree of AS in group C were lighter than group B;(2)The expression and activity about HO-1 in group D were decreased significantly than group C(P<0.05),the degree of AS in group D were lighter than group C.Conclusion CMN could induce HO-1 protein over expression and increase its activity and play an anti-AS role in rat’s aortic artery,however,this induction were blocked significantly by ZnPPⅨ.