严重精液异常冻融精子与新鲜精子进行卵胞浆内单精子注射后受精比较

目的了解严重精液异常的冻融精子与新鲜精子进行卵胞浆内单精子注射(ICSI)后受精比较。方法采用甘油-卵黄作为精子保护剂对15份再次取出的精液标本进行冻存。应用ICSI技术治疗,观察其受精、卵裂结果。在电子显微镜下扫描,观察冷冻前后精子的超微结构及其改变。结果冻融精子的受精率为62.7%,卵裂率为93.6%,新鲜精子的受精率为62.2%,卵裂率为94.5%,两者比较无显著性差异(P>0.05)。电镜扫描显示,新鲜精子头部结构完整,质膜、顶体及尾部均未见明显变化。冻融精子的改变,主要局限在其头部的质膜与顶体膜变皱,破裂甚至丢失,中段线粒体肿胀或破坏,基质密度降低等。结论精子冷冻复苏处理不影响ICSI后的受精率和卵裂率。

A comparison of frozen-thawed sperm to fresh sperm for fertilization by in-trocy to plasmic sperm injection

Objective To understand fertilizational and cleavage rates of th e frozen-thawed and fresh sperm by introcytoplasmic sperm injection(ICSI)in the severe abnormal semen.Method15semens with glycerol-yolk as sperm protective agent were adopted in cryopreservation,their insemination and cleavage result s were observed after the above sperms fretilized by ICSI.Ultrastructures and changes of the fresh and frozen-thzen-thawed specimen were observed under elec tron microscopy.Results The insemi-national and cleavage rates of frozen-thaw ed sperm were62.7%and93.6%;while the inseminational and cleavage rate s of fresh shoot sperm were62.2%and94.5%respec-tively.The results we re not showed statistical meaning(P>0.05),and showed that head structure was complete;membrane,acrosome and the filament of tail did not change ob-viously in the fresh sperm.The main changes of frozen-thawed sperm were that h ead menbrane and acrosomic membrane broke or lost,the mitochondrion swelled or destroyed and matrix density reduced.Conclusion The insemination and cleavage r ates after ICSI Can not be affected by frozen-thawed process.