The neuronal types and the distribution of 5-hydroxytryptamine and enkephalin-like immunoreactive fibers in the dorsal cochlear nucleus of the North American opossum

The opossum dorsal cochlear nucleus is divided into four layers distinguishable either on the basis of differential distribution of neuron types or by neuropil organization. We have used Nissl, Golgi and protargol stained preparations to examine these components. Four types of neurons (excluding granule cells) are seen. The principal neurons have large cell bodies arranged in a sheet defining layer II, their apical dendrites extend dorsally to form an elaborate arbor in layer I and their basal dendrites pass ventrally into layer III. Round cells are found throughout layers I and II. Their densely packed dendritic domains feature thick spine encrusted dendrites that have many recurrent branches. Giant neurons have large perikarya scattered throughout layers III and IV and long thick dendrites that radiate throughout the nucleus. Small multipolar neurons (stellate cells) are found throughout the nucleus. The more superficial ones have small perikarya whereas those found in deeper layers tend to be large. All four layers of the nucleus may be clearly differentiated in protargol stained sections. Layer I has small, thin fibers in parallel array, layer II has a mixture of fibers with an apparent random orientation, layer III has large diameter vertically oriented fibers, and layer IV has fibers of similar diameter but deposed horizontally. Immunohistochemical techniques have been used to identify specific fiber systems in the neuropil of the dorsal cochlear nucleus. Fibers containing 5-hydroxytryptamine (5-HT) immunoreactivity were prevalent in layers I, III and IV but sparse in layer II. Fibers containing enkephalin (ENK)-immunoreactivity were prevalent in layer I and II with only a few scattered fibers in the deeper layers. Isolated clusters of 5-HT and ENK immunoreactive fibers in layer II were found around principal neuron somata; similar clusters in the deep layers were located around the somata of giant neurons. The wide distribution of 5-HT immunoreactive fibers suggests they may be involved in a general regulation of activity in this nucleus; conversely the more circumscribed distribution of ENK immunoreactive fibers would suggest a restricted involvement of this fiber system with a specific feature of information processing.