| 扶正化瘀方与黄芪汤合用对实验性肝硬化大鼠的治疗作用 |
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| 引用本文： | 王骆冰,闫秀川,曾贞,吕靖,刘平,刘成海. 扶正化瘀方与黄芪汤合用对实验性肝硬化大鼠的治疗作用[J]. 中国中药杂志, 2010, 35(13): 1740-1744 |
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| 作者姓名： | 王骆冰 闫秀川 曾贞 吕靖 刘平 刘成海 |
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| 作者单位： | 1. 上海中医药大学附属曙光医院肝病研究所,上海,201203
2. 上海中医药大学附属曙光医院肝病研究所,上海,201203;上海高校中医内科学E-研究院,上海,201203 |
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| 基金项目： | 国家重点基础研究发展计划(973)项目(2006CB504801);国家自然科学基金项目(30772869);上海市优秀学科带头人计划项目(08XD14041);上海市教育委员会E-研究院建设计划项目(E0308);上海市教委创新团队建设项目 |
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| 摘 要： | 目的:观察扶正化瘀方与黄芪汤合用对实验性肝硬化大鼠的治疗作用,探讨补虚中药对肝硬化的治疗作用特点。方法:二甲基亚硝胺(DMN)腹腔注射4周制备肝硬化大鼠模型。成模后模型大鼠分别给予扶正化瘀方、黄芪汤、扶正化瘀方合黄芪汤灌胃2周。观察大鼠体重、肝重、脾重;HE染色观察肝组织炎症病理;天狼星红染色观察肝组织胶原沉积;试剂盒检测大鼠血清肝功能(ALT,Alb,T.Bil)、肝组织SOD活性及MDA含量;盐酸水解法测定肝组织Hyp含量;免疫组化染色观察肝组织α-SMA表达;Western blot检测肝组织α-SMA蛋白表达。结果:与正常大鼠相比,模型大鼠血清ALT,T.Bil明显升高,Alb含量显著降低;肝组织SOD活性下降,MDA和Hyp含量显著升高;肝组织炎症病变和胶原沉积明显,α-SMA表达显著增加。黄芪汤、扶正化瘀方及两方合用均可显著改善模型大鼠血清肝功能,改善肝脏脂质过氧化,降低肝组织Hyp含量,减轻肝组织炎症和胶原沉积,抑制α-SMA表达。在改善肝脏胶原沉积与抑制α-SMA表达方面,两方合用优于单用。结论:扶正化瘀方、黄芪汤、扶正化瘀方合黄芪汤均可改善DMN模型大鼠肝纤维化,两方合用较之各单方组抑制胶原沉积明显,其部分机制在于两方合用可更好抑制肝星状细胞活化。
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| 关 键 词： | 肝硬化 肝星状细胞 胶原 扶正化瘀方 黄芪汤 |
| 收稿时间： | 2009-11-19 |
Effect of Fuzheng Huayu recipe and Huangqi tang on DMN-inducedexperimental liver cirrhosis in rats |
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| WANG Luobing,YAN Xiuchuan,ZENG Zhen,LV Jing,LIU Ping and LIU Chenghai. Effect of Fuzheng Huayu recipe and Huangqi tang on DMN-inducedexperimental liver cirrhosis in rats[J]. China Journal of Chinese Materia Medica, 2010, 35(13): 1740-1744 |
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| Authors: | WANG Luobing YAN Xiuchuan ZENG Zhen LV Jing LIU Ping LIU Chenghai |
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| Affiliation: | Shanghai University of Traditional Chinese Medicine, Shuguang Hospital, Institute of Liver Diseases, Shanghai 201203, China;Shanghai University of Traditional Chinese Medicine, Shuguang Hospital, Institute of Liver Diseases, Shanghai 201203, China;Shanghai University of Traditional Chinese Medicine, Shuguang Hospital, Institute of Liver Diseases, Shanghai 201203, China;Shanghai University of Traditional Chinese Medicine, Shuguang Hospital, Institute of Liver Diseases, Shanghai 201203, China;Shanghai University of Traditional Chinese Medicine, Shuguang Hospital, Institute of Liver Diseases, Shanghai 201203, China;E-institute of Traditional Chinese Medicine, Shanghai Municipal Education Commission, Shanghai 201203, China;Shanghai University of Traditional Chinese Medicine, Shuguang Hospital, Institute of Liver Diseases, Shanghai 201203, China;E-institute of Traditional Chinese Medicine, Shanghai Municipal Education Commission, Shanghai 201203, China |
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| Abstract: | |
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| Keywords: | liver cirrhosis hepatic stellate cell collagen Fuzheng Huayu recipe Huangqi tang |
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