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Cardiotoxicity testing using pluripotent stem cell-derived human cardiomyocytes and state-of-the-art bioanalytics: a review |
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| Authors: | Mandenius Carl-Fredrik Steel Daniella Noor Fozia Meyer Thomas Heinzle Elmar Asp Julia Arain Sarina Kraushaar Udo Bremer Susanne Class Reiner Sartipy Peter |
| Affiliation: | 1. Biotechnology/IFM, Link?ping University, , Link?ping, Sweden;2. Cellartis AB, , G?teborg, Sweden;3. Biochemical Engineering, Saarland University, , Saarbruecken, Germany;4. MultiChannel Systems GmbH, , Reutlingen, Germany;5. Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, the Sahlgrenska Academy, University of Gothenburg, , G?teborg, Sweden;6. PerSens GmbH, , Regensburg, Germany;7. Natural and Medical Sciences Institute at the University of Tübingen, , Germany;8. ECVAM, Institute for Health and Consumer Protection (IHCP), European Commission Joint Research Center, , Ispra, Italy;9. Pharmacelsus GmbH, , Saarbruecken, Germany |
| Abstract: | In this article, recent progress in cardiotoxicity testing based on the use of immortalized cell lines or human embryonic stem cell (hESC) derived cardiomyocytes in combination with state-of-the-art bioanalytical methods and sensors is reviewed. The focus is on hESC-derived cells and their refinement into competent testing cells, but the access and utility of other relevant cell types are also discussed. Recent developments in sensor techniques and bioanalytical approaches for measuring critical cardiotoxicity parameters are highlighted, together with aspects of data evaluation and validation. Finally, recommendations for further research are given. |
| Keywords: | human embryonic stem cells in vitro toxicity cardiomyocyte cardiotoxicity cytotoxicity electrophysiology multielectrode array surface plasmon resonance respiration metabolomics |
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