新型牙周再生细胞传递载体的体外建立

目的 体外建立新型细胞传递载体，观察富血小板血浆（PRP）和贫血小板血浆（PPP）对鼠牙龈成纤维细胞、牙周膜成纤维细胞和牙槽骨成骨细胞在生物降解膜上附着及增殖的影响。方法 体外培养鼠牙龈成纤维细胞、牙周膜成纤维细胞和牙槽骨成骨细胞。应用梯度密度离心从鼠全血中获取PRP和PPP，并分别预处理生物降解膜。体外细胞培养48 h，检测鼠3种细胞在处理和未处理膜上的附着、增殖和形态。结果 鼠3种细胞在PRP和PPP处理膜上的附着数均较未处理组有显著增加（P<0.05）；鼠3种细胞在PRP处理膜上的附着数虽较PPP处理组略有增加，但统计学检验无意义（P＞0.05）；在同一处理或未处理组中，鼠3种细胞间附着数比较无统计学意义（P＞0.05）。体外培养1 h和48 h，鼠3种细胞在PRP和PPP处理膜上的增殖均显著强于未处理组（P<0.05）；体外培养48 h，鼠3种细胞在PRP和PPP处理膜上的增殖显著强于体外培养1 h（P<0.05）；在同一处理或未处理组中，鼠3种细胞间的增殖则无统计学意义（P＞0.05）。附着于PRP和PPP处理膜上的鼠3种细胞均不同程度显示胞体增大，胞浆扩展，有的形成丝状伪足。结论 PRP和PPP处理的生物降解膜能明显加强鼠各型细胞的附着和增殖，这为建立细胞传递载体和促进牙周再生奠定实验基础。

Development of Novel Cell Delivery Carrier for Periodontal Regeneration in vitro

Objective To investigate the development of novel cell delivery carriers and evaluate the effects of platelet- rich plasma( PRP)and platelet- poor plasma( PPP)on cellular attachment and proliferation. Methods Rat gingival fibroblasts, periodontal ligament fibroblasts and osteoblasts were cultured in vitro. The biodegradable mem- branes were treated with PRP and PPP that were obtained from rat whole blood by gradient density centrifugation. The cellular attachment, proliferation and morphology on the untreated membranes and membranes treated with PRP and PPP were tested in vitro for 48 hours. Results There were significant differences between the untreated mem- branes and the membranes treated with PRP and PPP for three kinds of rat cells used in the experiment. There were no significant differences between those membranes treated with PRP and those treated with PPP. Three kinds of cells on the membranes treated with PRP were slight higher than those on the membranes treated with PPP. There were no significant differences in the same group for cells attachment. The rate of different cells proliferation on the untreated membranes was significant lower than those on the membranes treated with PRP and PPP at 1 hour and 48 hours after seeding. At the same time, the rate of different cells proliferation on the treated membranes at 48 hours was significant higher than those on the treated membranes at 1 hour. There was no significant differ- ences in the same group for the rate of cells proliferation. Most of the cells attached on the membranes treated with PRP and PPP exhibited robust and elongated forms with numerous filopodial extensions. Conclusion The results showed that the biodegradable membranes treated with PRP and PPP could promote rat cell attachment and prolifer- ation. It suggests that this novel technique will provide the experimental basis for the development of cell delivery carriers and periodontal regeneration.