| 组织工程牙周膜细胞片的体内实验研究 |
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| 引用本文： | 王方,谢诚,吴国锋,赵铱民. 组织工程牙周膜细胞片的体内实验研究[J]. 实用口腔医学杂志, 2011, 27(2): 177-180. DOI: 10.3969/j.issn.1001-3733.2011.02.06 |
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| 作者姓名： | 王方 谢诚 吴国锋 赵铱民 |
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| 作者单位： | 第四军医大学口腔医学院口腔修复科,西安,710032 |
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| 摘 要： | 目的:探索一种形成组织工程牙周膜的新方法并建立动物模型,为牙周组织工程奠定基础。方法:原代分离培养人牙周膜细胞,部分细胞复合明胶海绵设为对照组;另一部分细胞经特殊条件连续培养成细胞膜片设为实验组。2组实验结果择优复合支架材料后植入裸鼠皮下,4周后取材行组织病理检测。结果:经特殊条件连续培养的牙周膜细胞层叠交错相连形成具有良好的机械加工性能的生物膜片,该膜片经裸鼠体内培养形成牙周膜样组织,并在近羟基磷灰石支架侧有矿化的牙骨质样结构生成。结论:该方法制备的牙周膜细胞片在牙周组织工程具有良好的应用前景。
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| 关 键 词： | 组织工程 牙周膜 纳米羟基磷灰石 细胞膜片 |
An in vivo study of cell sheet technique for periodontal tissue engineering |
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| WANG Fang,XIE Cheng,WU Guofeng,ZHAO Yimin. An in vivo study of cell sheet technique for periodontal tissue engineering[J]. Journal of Practical Stomatology, 2011, 27(2): 177-180. DOI: 10.3969/j.issn.1001-3733.2011.02.06 |
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| Authors: | WANG Fang XIE Cheng WU Guofeng ZHAO Yimin |
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| Affiliation: | WANG Fang,XIE Cheng,WU Guofeng,ZHAO Yimin.710032 Xi'an,Department of Prosthetic Dentistry,School of Stomatology,The Fourth Military Medical University,China |
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| Abstract: | Objective: To develop a new way of preparation of periodontal ligament cell(PDLC) sheet and establish an animal test model.Methods: Primarily cultured human PDL cells were seeded on the surface of gelfoam as the control group,and those continuously cultured in perti dishes as the experimental group.In control group no cell sheet was formed,but in experimental group cell sheets were developed.Then,the cell sheets were used to cover hydroxyapatite(HA) scafford and transplanted under the skin of immunocompromi... |
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| Keywords: | Tissue engineering Periodontal ligament Nano-HA Cell sheet |
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