印迹基因PEG10在胃腺癌组织中的表达及意义

目的：研究印迹基因PEG10在胃癌及癌旁组织中的表达情况，探讨其对胃癌细胞生长的影响。方法：采用逆转录聚合酶链反应（RT-PCR）检测40例胃癌、癌旁组织及6例正常胃组织中印迹基因PEG10 mRNA表达；构建表达PEG10质粒，并将其转染到不表达内源性PEG10的胃癌细胞系MKN45中，应用噻唑蓝比色分析法（MTT），测定转染PEG10后MKN45细胞的生长情况。结果：20例胃癌组织中9例（45.0%）PEG10表达阳性，对应的癌旁组织仅有2例（10.0%）表达阳性，2组间PEG10表达率比较差异有显著性(Ｐ<0.05)，正常胃组织无PEG10表达；胃癌细胞系MKN45无内源性PEG10表达，经质粒转染PEG10基因后的MKN45细胞的生长速度加快。结论：印迹基因PEG10在胃癌组织中高表达，具有促进胃癌细胞生长的作用。

Expression of imprinted gene PEG10 in human gastric adenocarcinoma tissues and significance

Objective To investigate the expression of a novel imprinted gene,PEG10,in human gastric adenocarcinoma,and the effect of PEG10 on cell growth and proliferation of gastric cancer cells.Methods The PEG10 mRNA expressions in 40 human gastric adenocarcinoma,the corresponding adjacent normal tissues and 6 nomal gastric tissues were detected by RT-PCR.The expression vectors of PEG10 were constructed and transfected into gastric cancer cell line MKN45 which had no endogenetic PEG10 expression.Cell growth ability was measured by MTT assay.Results High PEG10 mRNA expression level was detected in 9 of 20(45.0%) human gastric adenocarcinoma which was significantly higher than those of the matched normal tissues(10.0%)(P<0.05).No expression of PEG10 in nomal gastric tissues and gastric cancer cell line MKN45.The growth ability of transfected MKN45 cells was increased.Conclusion The imprinted gene PEG10 can overexpress in human gastric adenocarcinoma and enhance the growth ability of gastric cancer cells.