扶正化瘀方对骨髓移植肝纤维化小鼠骨髓细胞肝脏迁移的影响

目的: 探讨扶正化瘀方(FZHY)对肝纤维化小鼠骨髓细胞肝脏归巢的作用。 方法: BABL/C雌性受鼠性别交叉法骨髓移植模型基础上,四氯化碳(CCl₄)诱导肝纤维化模型;成模后,治疗组以FZHY方4.6 g ·kg^-1 ·d^-1 ig 4周。 PCR,病理及肝功能检测成模情况;通过FISH法标记Y染色体,观察雌性受鼠肝脏内骨髓源性细胞含量;Western blot法检测雌性受鼠肝组织基质细胞衍生因子-1(SDF-1)蛋白相对表达量。 结果: 与模型组比较,FZHY组小鼠,肝组织损伤明显减轻。FISH法标记Y染色体观察显示,FZHY组小鼠较模型组肝内骨髓源性细胞减少(P<0.05)。Western blot结果,FZHY组小鼠较模型组肝脏SDF-1蛋白相对表达量明显减少(P<0.01)。 结论: 在肝脏损伤时,FZHY可能通过抑制肝内SDF-1蛋白的表达来阻止骨髓源性细胞迁移入肝,从而一定程度上抑制骨髓源性细胞入肝后促进肝纤维化。

Effects of Fuzheng Huayu Decoction on Marrow Cells Migrating into Liver in Bone Marrow Transplanted Mice Fibrosis Liver

Objective : The discussion straightened up the effects of the Fuzheng Huayu (FZHY) decoction on the marrow cells migrating into liver in mice fibrosis liver. Method: Based on the model of the transposition bone marrow between female and male BABL/C mice, we duplicated the CCl₄ liver fibrosis mice model, and then the mice in FZHY group were administered with FZHY decoction ig for 4 weeks. Did PCR, liver function and pathology examination to view the model., marked the Y through the FISH to observe the number of the cells from the male mice bone marrow in female mice livers, tested the chemokine SDF-1 relative expression in female mice livers by Western blot. Result: The damage of mice liver relieved obviously in FZHY decoction group compared the model group. The observation of Y chromosome dyed indicated, the number of the cells from the male mice bone marrow reduced in the FZHY decoction group compared the model group liver(P<0.05). The chemokine SDF-1 relative expression reduced in the FZHY decoction group compared the model group liver (P<0.01). Conclusion: When the liver is damaged, the FZHY decoction may be able to depress the chemokine SDF-1 expression to prevent the marrow cells to migrate into the liver, and thus in certain degree controls the marrow cell promoting the liver fibrosis.