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| α粒子体外诱发叙利亚地鼠胚胎细胞恶性转化 | |
| 引用本文： | 寿 江,龚诒芬,章扬培,刘秀林,吴德昌. α粒子体外诱发叙利亚地鼠胚胎细胞恶性转化[J]. 中国药理学与毒理学杂志, 1998, 12(3): 201-206 |
| 作者姓名： | 寿 江 龚诒芬 章扬培 刘秀林 吴德昌 |
| 作者单位： | 北京放射医学研究所 |
| 摘 要： | 叙利亚地鼠胚胎细胞经２００ｃＧｙα粒子（５．３４ＭｅＶ）照射后连续传代．传至２０代时，用低密度培养法分离出１１个克隆，采用多项指标观察其生物学特性的变化，最后经ＮＩＨ３Ｔ３细胞转染和裸鼠接种试验证实，第２０代细胞和Ｔ４克隆细胞具有恶性转化细胞的特征． |
| 关 键 词： | α粒子；叙利亚地鼠；胚胎；细胞转化.恶性；基因 |
| 收稿时间： | 1996-10-17 |
Characterization of transformed SHE cells by alpha particles in vitro |
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| SHOU Jiang, GONG Yi-Fen, ZHANG Yang-Pei, LIU Xiu-Lin, WU De-Chang. Characterization of transformed SHE cells by alpha particles in vitro[J]. Chinese Journal of Pharmacology and Toxicology, 1998, 12(3): 201-206 | |
| Authors: | SHOU Jiang GONG Yi-Fen ZHANG Yang-Pei LIU Xiu-Lin WU De-Chang |
| Affiliation: | (Institute of Radiation Medicine, Beijing 100850) |
| Abstract: | Eleven monoclones were isolated from the primary Syrian hamster embryo (SHE) cells at passage 20 following alpha-irradiation at a dose of 200 cGy, 5.34 MeV. Cells at the 20 th passage and isolated survived clones were examined for their oncogenic characters. Using soft agar assay, chromosome aberration analysis, genomic transfection and tumorigenicity assay in nude mice, cells of the clone T4 and at passage 20 were confirmed to be oncogenic. |
| Keywords: | alpha particles hamster Syrian embryo cell transformation neoplastic genes |
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