玻璃化冷冻胚胎移植对小鼠子代发育和学习记忆能力的影响

目的:初步探讨小鼠胚胎玻璃化冷冻对子代发育及学习记忆能力的影响.方法:昆明雌性小鼠106只超排卵受孕后,取出胚胎分为2组,冷冻组采用玻璃化冷冻方法冷冻优质胚胎816枚,并于2周后复融、移植;对照组移植新鲜优质胚胎752枚.将2组胚胎移植入假孕小鼠体内.记录2组仔鼠出生第0、3、7、14、21、28、60天的体质量,观察耳廓分离、上下牙萌出、张耳、睁眼、全身绒毛、全身白毛、雄性仔鼠睾丸下降、雌性仔鼠阴道开口等生长发育指标达标时间,测试平面翻正、前肢悬挂、爬行、空中翻正等神经反射和运动协调功能达标时间,出生后60 d对仔鼠进行迷宫实验.结果:仔鼠体质量逐渐增加,2组同一时间点比较,差异无统计学意义(F时间=12.021,P=0.001;F组间=0.187,P=0.163).耳廓分离、上下牙萌出、张耳、睁眼、全身绒毛、全身白毛、雄性仔鼠睾丸下降、雌性仔鼠阴道开口等达标的时间,测试平面翻正、前肢悬挂、爬行、空中翻正等达标的时间,以及学习记忆能力比较,差异均无统计学意义(P＞0.05).结论:玻璃化冷冻对子代小鼠生长发育和记忆功能无明显影响.

Effect of vitrification on development and memory of offspring in mice

Aim:To assess the development and memory of offspring in mice from cryopreservation by vitrification.Methods:Embryos derived from 106 mice after pregnanting through over-ovulation.The selected embryos were divided into two groups,one was control group including 752 embryos,and the other was vitrified group(vitrified embryos were stored for 2 weeks in liquid nitrogen,then thawed and transferred) including 816 embryos,which were then transferred to uterus of pseudopregnant mice.The offspring was 99 and 103 in control and vitrifed group,respectively.The development and memory of live pups after natural delivery in the two groups were compared.Results:The average weight of newborn,the physical development and movement coordination function,and T-maze test results between the 2 groups had no significant differences(P>0.05).Conclusion:Vitrification had less impact on the development and memorial ability of mice pups.