IL-19及其相关因子在大鼠自身免疫性心肌炎各时程的表达特征

目的探讨IL-19及其相关因子在大鼠实验性自身免疫性心肌炎(EAM)急慢性期各时程的表达特征。方法建立大鼠EAM模型;病理学评估EAM急慢性期(normal、3w、3m、6m)心肌损伤程度;应用实时荧光定量RT-PCR检测IL-19及其两条受体链IL-20R1/IL-20R2,IL-20在EAM大鼠各脏器(心、肝、脾、肾)以及在心肌组织各时程(normal、3w、3m、6m)的mRNA表达;进一步应用ELISA法检测IL-19和IL-20在EAM各时程心脏组织匀浆中的蛋白表达。结果在EAM大鼠急性期3周时IL-19、IL-20与IL-20R2主要在心脏表达,IL-20R1主要在肾脏表达;在EAM大鼠慢性期3个月时IL-19主要在脾脏表达,IL-20主要在肝脏及脾脏表达,IL-20R1主要在肾脏表达,IL-20R2主要在心脏表达;IL-19及其两条受体链IL-20R1/R2在EAM大鼠心肌组织的表达高峰均出现在急性期3周,随后逐渐减少,在慢性期(3m、6m)已基本接近正常水平;而IL-20在EAM大鼠心肌组织的表达高峰在慢性期3个月,IL-19和IL-20心肌组织的蛋白水平与基因表达呈现一致性。结论 IL-19及其相关因子参与EAM的发病进程,IL-19主要在EAM急性期炎症反应的过程中发挥重要作用。

Time course of IL-19 and related factors expression in rat experimental autoimmune myocarditis

We aim to explore the time course of IL-19 and its related factors expression in rat experimental autoimmune myocarditis(EAM).The EAM model was constructed and the degree of myocardium injury at acute phase and chronic phase of EAM(normal,3 weeks,3 months,6 months) was evaluated by histopathology.The mRNA expressions of IL-19 and related factors in different tissues(heart,liver,spleen,kidney) and different time course of heart tissue(normal,3 week,3 months,6 months) from EAM rats were determined by quantitative real-time RT-PCR.Furthermore,time course of IL-19 and IL-20 protein expression of heart tissue homogenate from EAM were determined by ELISA.The results indicated at acute phase EAM of week 3,the IL-19,IL-20 and IL-20R2 mRNA mainly expressed in heart,while IL-20R1 mainly expressed in kidney.At chronic phase EAM of month 3,IL-19 mainly expressed in spleen,IL-20 expressed in liver and spleen,IL-20R1 expressed in kidney,while IL-20R2 mainly expressed in heart.The time course showed that IL-19 and its receptors complex were mainly expressed at the acute phase EAM of week 3,while IL-20 mainly expressed at the chronic phase EAM of month 3.The expressions of IL-19 and IL-20 protein in heart tissue were corresponded to gene expression.This study might prove that IL-19 and related factors are involvement in the pathologyical process of EAM,and IL-19 may play an important role in acute phase of EAM rats.