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| 共刺激分子HVEM在类风湿关节炎滑膜组织内的表达及分布研究 | |
| 引用本文： | 尚永军,崔其福,李金龙,郭国宁. 共刺激分子HVEM在类风湿关节炎滑膜组织内的表达及分布研究[J]. 免疫学杂志, 2012, 0(6): 502-505,509 |
| 作者姓名： | 尚永军 崔其福 李金龙 郭国宁 |
| 作者单位： | 内蒙古赤峰学院第一附属医院骨科;第三军医大学附属西南医院急诊科 |
| 基金项目： | 国家自然科学基金资助项目(60971117、30700855、61141012) |
| 摘 要： | 目的探讨TNF家族共刺激分子HVEM(herpesvirus entry mediator)在类风湿关节炎(RA)患者滑膜组织内的表达及分布。方法使用免疫组化法检测RA患者滑膜组织HVEM的表达;并使用免疫荧光法检测HVEM的细胞定位及分布。结果免疫组化结果证实,RA患者滑膜组织中有大量的HVEM阳性细胞,形态观察提示这些阳性的细胞主要是毛细血管、滑膜细胞、局部淋巴结T细胞及巨噬细胞;免疫荧光分析进一步表明这些HVEM+细胞主要为CD3+T细胞,CD68+巨噬细胞,少数CD31+内皮细胞也表达HVEM,但其不表达于CK-18+上皮细胞。对比其他B7家族共刺激分子在滑膜组织中的分布,免疫荧光发现HVEM共表达于B7-H3+血管,但不表达于B7-H1+、B7-DC+、B7-H4+及Z39Ig+细胞。结论关节炎滑膜组织内有大量HVEM阳性细胞,提示HVEM有可能参与并调节了关节炎的病理进程。 |
| 关 键 词： | HVEM 类风湿关节炎 滑膜组织 免疫组化 |
Expression and distribution of the costimulatory molecule HVEM in synovial tissues of rheumatoid arthritis |
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| SHANG Yongjun,CUI Qifu,LI Jinlong,GUO Guoning. Expression and distribution of the costimulatory molecule HVEM in synovial tissues of rheumatoid arthritis[J]. Immunological Journal, 2012, 0(6): 502-505,509 | |
| Authors: | SHANG Yongjun CUI Qifu LI Jinlong GUO Guoning |
| Affiliation: | Department of Orthopedics,First Affiliated Hospital of Chifeng University,Chifeng 024000,China |
| Abstract: | The expression of HVEM(herpesvirus entry mediator) antigen has been detected on many inflammation diseases.Here we aimed to evaluate the expression of HVEM in rheumatoid arthritis(RA) synovium.Immunohistochemical analysis revealed that HVEM positive cells were found in RA synovium,and fluorescence double-staining further demonstrated that HVEM positive cells were major on CD3+ T cells,CD68+ macrophages,occasional was found on CD31+ endothelial cells,while it was absence on CK-18+ epithelial cells.In contrast to other members of B7 superfamily,the expression of HVEM was also found on B7-H3+ capillary,but absent on B7-H1+,B7-DC+,B7-H4+ or CRIg-positive cells.All the result suggested that HVEM signal might be involved in regulating local T cell activation and finally participated in the pathogenesis of RA. |
| Keywords: | HVEM Rheumatoid arthritis Synovial tissues Immunohistochemistry |
| 本文献已被 CNKI 等数据库收录！ | |