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A monoclonal antibody that recognizes an antigenic determinant shared by HLA A2 and B17
Authors:Andrew J. McMichael  Peter Parham  Nigel Rust  Frances Brodsky
Affiliation:1. Nuffield Department of Medicine, University of Oxford, Oxford, U.K.;2. Department of Biochemistry, University of Oxford, Oxford, U.K.;3. Biological Laboratories, Harvard University, Boston, Massachusetts, USA
Abstract:
A hybridoma monoclonal anti-HLA antibody has been produced by the technique of Kobler and Milstein [1]. This antibody recognizes a new specifity common to HLA A2 and B17. It was shown to be a single antibody by isoelectric focusing and absorption experiments.
Keywords:FCS  fetal calf serum  T  thymidine  BSA  bovine serum albumin  SDS  sodium dodecyl sulphate  PBS  phosphate-buffered saline  DTT  dithiothreitol  H  hypoxanthine  DMSO  dimethysulphoxide  A  aminopterin
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