| 人表皮生长因子真核表达载体的鉴定 |
| |
| 引用本文： | 张立华,银平章,鲍玉洲.人表皮生长因子真核表达载体的鉴定[J].实用诊断与治疗杂志,2004,18(2):104-106. |
| |
| 作者姓名： | 张立华 银平章 鲍玉洲 |
| |
| 作者单位： | 450003,郑州市,河南省人民医院病理科 |
| |
| 摘 要： | 目的：鉴定人表皮生长因子的真核表达载体。方法：重组体进行限制性酶切及PCR可见目的片段。结果：pCDDA3.1—hEGF真核表达载体已成功构建。结论：已成功构建hEGF真核表达裁体，为进一步研究hEGF的功能奠定了基础。
|
| 关 键 词： | 人表皮生长因子 真核表达载体 基因转染 hEGF PCR 基因序列 |
| 文章编号： | 1672-3457(2004)02-104-03 |
| 修稿时间： | 2003年11月20 |
Identification of eukaryotic expression vector of human epidermal growth factor |
| |
| ZHANG Lihua,YIN Pingzhang,BAO Yuzhou.Identification of eukaryotic expression vector of human epidermal growth factor[J].Journal of Practical Diagnosis and Therapy,2004,18(2):104-106. |
| |
| Authors: | ZHANG Lihua YIN Pingzhang BAO Yuzhou |
| |
| Institution: | ZHANG Lihua,YIN Pingzhang,BAO Yuzhou. Department of Pathology,Henan Provincial People's Hospital,Zhengzhou 450003,China |
| |
| Abstract: | ? Objective To identify eukaryotic expression vector of human epidermal growth factor.Methods Restriction endonuclease and polymerase chain reaction were used to identify eukaryotic expression vector pcDNA3.1-hEGF. Results The eukaryotic expression vector of human epidermal growth factor had been successfully constructed. Conclusion The successful construction of EGF will lay the foundation for future reseach. |
| |
| Keywords: | Human epidermal growth factor eukaryotic expression vector gene transfection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
