舌下神经核神经元内白细胞介素2受体、孕激素受体和谷氨酸受体共存

为了探讨一个神经元同时受到免疫，神经内分泌物质调节的可能性，在大鼠舌下神经核的两张相邻切片上一张切片用PAP法与SABC法相结合，显示白细胞介素2受体（IL-2R0和孕激素受体（PR），另一张用SABC法显示代谢型谷氨酸5亚型受体（mGluR5)。结果表明，在舌下神经核的IL-2R/PR双标切片上，可见3类阳性反应细胞，IL-2R免疫反应单标细胞，PR免疫反应单标细胞，IL-2R/PR免疫反应双标细胞，后者约占舌下神经核阳性反应细胞的45%，在另一张相邻的显示mGluR5的切片上，有较多的免疫反应阳性细胞，将两张相邻切片的照片进行对合检查证明，在大鼠舌下神经核存在IL-2R/PR/mGluR5三类物质受体共存的神经元，它们约占IL-2R/PR双标细胞总数的25%，提示免疫，神经，内分泌物质可能通过共存于同一神经元上的相应受体共同调节神经元的功能。

Coexistence of Interleukin-2 Receptor, Progesterone Receptor and Metabotropic Glutamate Receptor Subunit 5 in Neurons of Hypoglossal Nucleus

In order to explore the possibility that the neuron was simultaneously regulated by immune neuro endocrine substances, interleukin 2 receptor (IL 2R), progesterone receptor (PR) and metabotropic glutamate receptor subunit 5 (mGluR 5) in hypoglossal nucleus of rats were investigated by immunohistochemical staining technique (two adjacent sections, one for IL 2R and PR, another for mGluR 5 ). The method of PAP combined with SABC was used in double immunocytochemical staining section for IL 2R and PR and the SABC method was used for mGluR 5 single labeling section. The results showed that there were three kinds of immunoreactive (ir) positive cells: IL 2R ir single labeled cells, PR ir single labeled cells, IL 2R/PR ir double labeled cells which accounted for about 45 % of the labeled cells in IL 2R/PR double labelling section. In another adjacent section, there were many mGluR 5 ir positive cells. It was identified that there was coexistence of IL 2R/PR/mGluR 5 in same neurons of hypoglossal nucleus of rat by the corresponding check of the photos of two adjacent sections. The neurons with coexistence of the three receptors accounted for about 25 % of total IL 2R/PR double labeled neurons. The results suggest that immune neuro endocrine substances might commonly regulate neuronal function by corresponding receptors which coexisted in same neurons.