硒蛋白S基因多态性与乳头状甲状腺癌发病风险的关联性

目的 探讨硒蛋白S（SelS）基因（rs34713741位点）基因多态性与乳头状甲状腺癌（PTC)发病风险的相关性。方法 采用等位基因特异性PCR（AS - PCR）技术检测138例PTC患者和140例健康对照者SelS基因（rs34713741位点）基因型和等位基因分布频率，并根据不同临床特征（年龄、性别、肿瘤大小和有无淋巴转移）进行分层分析，同时结合非遗传相关因素（吸烟、饮酒、BMI、碘盐摄入、精神状态等）联合分析该位点SelS基因多态性与PTC发病风险的关联性。结果 BMI、碘盐摄入、工作压力是PTC发病风险因素，精神状态是PTC保护性因素（P均＜0.1）。该位点SelS基因CT、CC、TT基因型和C、T等位基因分布频率在2组间无统计学意义（χ2分别为3.404，3.258，P均＞0.05）。分层分析显示:女性组、无淋巴转移组CC、CT、TT基因型分布与对照组相比存在统计学差异（χ2 = 4.430，P = 0.035，；χ2 = 3.967，P = 0.046），PTC女性组、无淋巴转移组TT基因型明显高于对照组（OR = 1.554，95%CI:1.032～2.344；OR = 1.457，95%CI:1.006～2.109）。结论 PTC发病与BMI、碘盐摄入、精神状态和工作压力等相关，T等位基因可能是女性PTC发病因素之一，同时也是无淋巴转移保护性因素之一。

Association between selenoprotein S polymorphism and the risk of papillary thyriod carcinoma

Objective To investigate the association between selenoprotein S(SelS) rs34713741 polymorphism and papillary thyriod carcinoma(PTC). Methods The genotype and allele frequency of the SelS from 138 patients with PTC and 140 healthy controls were determined by allele-specific PCR(AS-PCR) and were confirmed by sequencing analysis. Stratified analysis was conducted according to different clinical characteristics(age, gender, tumor size and presence or absence of lymph node metastasis), and the association of the SelS gene polymorphism with PTC incidence risk was analyzed in combination with non-genetic factors(smoking, drinking, BMI, iodized salt intake, mental state, etc.). Results BMI, iodized salt intake and work pressure are risk factors of PTC, while mental state is protective factor of PTC(P both＜0.1). The differences in the frequencies of CC, CT, and TT genotypes and C, T alleles of SelS rs34713741 between PTC patients and healthy controls were nonsignificant(χ2=3.404, 3.258, P both>0.05). But the stratification analysis results showed that there were significant differences of the genotype frequency of rs34713741 site between PTC group and control group in the women patients and no lymphatic metastasis patients(χ2=4.430, P=0.035; χ2=3.967, P=0.046). and the genotype of TT of the patients in PTC was significantly both higher than that in control group(OR=1.554, 95%CI: 1.032-2.344; OR=1.457, 95%CI: 1.006-2.109). Conclusion The incidence of PTC is related to BMI, iodized salt intake, mental state and work pressure.Allele T may be a genetic risk factor for women, also may be the protective factor that reduce the lymphatic metastasis of PTC.