MAGE-3抗原肽致敏DC疫苗抗膀胱癌细胞的体外实验

目的探讨黑色素瘤MAGE-3抗原肽致敏的树突状细胞(DC)疫苗体外抗膀胱癌细胞增殖作用及其分子机制。方法采用Ficoll法从HLA-A2型外周血中分离单核细胞,细胞因子GM-CSF和白细胞介素4(IL-4) 诱导扩增DC细胞后经MAGE-3抗原肽致敏,致敏DC细胞和同型T淋巴细胞共培养法诱导细胞毒性T淋巴细胞(CTL)。MTT法检测抗原肽致敏DC对T淋巴细胞增殖的影响,CTL对靶细胞BIU-87的杀伤作用。采用流式细胞术检测肿瘤细胞凋亡和凋亡相关蛋白变化。结果MAGE-3抗原肽致敏的DC对T淋巴细胞增殖率具有明显提高作用,高于无关抗原致敏DC及未致敏DC。经MAGE-3九肽冲击的DC可有效诱导对MAGE-3阳性细胞BIU-87杀伤的CTL活性,而无关抗原肽及未经抗原肽致敏的DC则不具备其杀伤能力。结论MAGE-3九肽致敏DC能显著促进T淋巴细胞增殖并诱导有效杀伤靶细胞的CTL作用。

The MAGE-3-sensitized dendritic cell vaccine kills bladder cancer cells in vitro

Objective To explore the cytotoxic activity of cytotoxicity T lymphocyte(CTL) induced by MAGE-3-sensitized dendritic cells(DCs) on the bladder cancer cell line BIU-87.Methods Proliferation of T cells and cytotoxic activity of CTL were determined by the MTT method.CTL was induced by MAGE-3-sensitized DC cells,and ovalbumin(OVA) peptide and un-sensitized DC served as controls.Results The MAGE-3-sensitized DC could promote proliferation of T cells and cytotoxity activity of the CTL cells on bladder cancer cells as compared with OVA peptide or unsensitized DC.Conclusion DC sensitized by the MAGE-3 antigen peptide can promote multiplication of T cells and can develop a cell immunity function,it also inhibits the growth of tumor and accelerates tumor decline.It can be used as a novel vaccine on bladder cancer therapy.