| 脑通复方制剂对血管性痴呆模型大鼠海马神经元细胞外调节蛋白激酶表达的影响 |
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| 引用本文： | 肖雁,吴昌学,周凡,齐晓岚,李毅,况时祥,黄勇,王永林,官志忠. 脑通复方制剂对血管性痴呆模型大鼠海马神经元细胞外调节蛋白激酶表达的影响[J]. 中国医院药学杂志, 2011, 0(9) |
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| 作者姓名： | 肖雁 吴昌学 周凡 齐晓岚 李毅 况时祥 黄勇 王永林 官志忠 |
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| 作者单位： | 贵阳医学院分子生物学重点实验室;贵阳中医学院附属第二医院神经科;贵阳医学院药学系; |
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| 基金项目： | 国家自然科学基金资助项目(编号:30870986); 贵州省中药现代化专项资助项目[编号:黔科合农字(2006)5023]; 贵阳医学院博士启动基金(编号:200913) |
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| 摘 要： | 目的:探讨观察脑通复方制剂对拟血管性痴呆(VaD)模型大鼠海马神经元中分裂原激活的蛋白激酶(MAPK)信号转导系统细胞外调节蛋白激酶(ERK1/2)及其磷酸化(p-ERK1/2)表达的影响。方法:采用改良Pulsinelli四血管阻断法复制大鼠血管性痴呆模型,脑通复方制剂治疗后观察学习记忆能力,Western-blotting法测定海马神经元总ERK1/2及p-ERK1/2蛋白表达。结果:脑通复方制剂可缩短实验大鼠逃避潜伏期,增加跨越平台次数;假手术组、VaD模型组、脑通复方制剂组及多哌齐特(安立申)组的ERK1/2蛋白水平分别为(1.20±0.19)、(1.11±0.14)、(1.18±0.13)、(1.25±0.14),任意两组间比较,差异均无统计学意义(P>0.05);假手术组、VaD模型组、脑通复方制剂组及多哌齐特(安立申)组p-ERK1/2蛋白水平分别为(0.75±0.07)、(0.38±0.08)、(0.58±0.10)、(0.65±0.08),模型组与假手术组相比p-ERK1/2蛋白表达降低,差异有统计学意义(P<0.01);脑通复方制剂组和多哌齐特(安立申)组p-ERK1/2蛋白表达较模型组升...
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| 关 键 词： | 血管性痴呆 细胞外调节蛋白激酶 脑通复方制剂 |
Effect of Naotong compound recipe on the changes of ERK in the hippocampal neurons in vascular dementia rats |
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| XIAO Yan,WU Chang-xue,ZHOU Fan,QI Xiao-lan,LI Yi,KUANG Shi-xiang,HUANG Yong,WANG Yong-lin,GUAN Zhi-zhong. Effect of Naotong compound recipe on the changes of ERK in the hippocampal neurons in vascular dementia rats[J]. Chinese Journal of Hospital Pharmacy, 2011, 0(9) |
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| Authors: | XIAO Yan WU Chang-xue ZHOU Fan QI Xiao-lan LI Yi KUANG Shi-xiang HUANG Yong WANG Yong-lin GUAN Zhi-zhong |
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| Affiliation: | XIAO Yan1,WU Chang-xue1,ZHOU Fan1,QI Xiao-lan1,LI Yi1,KUANG Shi-xiang2,HUANG Yong3,WANG Yong-lin3,GUAN Zhi-zhong1(1.The Key Lab of Medical Molecular Biology,Guiyang Medical College Guiyang,Guizhou Guiyang 550004,China,2.Department of Neurology,the Second Affiliated Hospital of Guiyang Traditional Chinese Medical College,Guizhou Guiyang 550003,3.Department of Pharmacy,Guiyang Medical College,China) |
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| Abstract: | OBJECTIVE To observe the effect of Naotong compound recipe on the changes of extracellular signal-regulated kinase(ERK and p-ERK) in the hippocampal neurons in vascular dementia(VaD) rat.METHODS The VaD model rats were established with modified 4-VO.All the experimental rats were divided into Naotong compound recipe,model, donepezil hydrochloride and sham operation groups.The protein levels of ERK1/2 in rat hippocampus were detected by Western-blotting.RESULTS The ERK1/2 protein levels in sham operation,mod... |
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| Keywords: | vascular dementia(VaD) extracellular signal-regulated protein kinase learning naotong compound recipe |
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