HPLC法同时测定舒胸速释微丸中4种化学成分的含量

目的建立同时测定舒胸速释微丸中羟基红花黄色素A、阿魏酸、三七皂苷R1和人参皂苷Rg14种化学成分含量的高效液相色谱法。方法采用Kromasil C18柱(250 mm×4.6 mm,5μm);以乙腈(A)和质量分数为0.05%的磷酸水溶液(B)作流动相,梯度洗脱;柱温:30℃;流速:1.0 mL.min-1;检测波长:203 nm。结果羟基红花黄色素A、阿魏酸、三七皂苷R1和人参皂苷Rg1的线性范围分别为1.88~37.50 mg.L-1(r=0.999 7),0.91~18.20 mg.L-1(r=0.999 5),2.25~45.00 mg.L-1(r=0.999 5)和15.10~302.00 mg.L-1(r=0.999 8),平均加样回收率(n=9)分别为97.3%、97.8%、98.7%和98.5%,RSD为1.8%、1.5%、1.4%和1.8%。结论HPLC法可为舒胸速释微丸提供含量测定的方法。

Determination of the content of four kinds of components in Shuxiong tinypill by HPLC

Objective To determine four bioactive component in Shuxiong tinypill(traditional Chinese medicines),namely hydroxysafflower yellow A,ferulic acid, notoginsenoside R1 and ginsenoside Rg1 quantitively.Methods The sepearation was performed on a Kromasil C18 column （250 mm×4.6 mm,5 μm）with the mobile phase consisting of acetonitrile-0.05%(w) phosphoric acid for gradient elution.The flow rate was 1.0ml·min-1 and the column temperature was set at 30 ℃.Results This method was applied to determination 4 bioactive components in Shuxiong tinypill successfully.The standard curves were linear over the rages of 1.88-37.50 mg·L-1 for hydroxysafflower yellow A （r=0.999 7）,0.91-18.20 mg·L-1 for ferulic acid（r=0.999 5）, 2.25-45.00 mg·L-1 for notoginsenoside R1（r=0.999 5）,15.10-302.00 mg·L-1 for ginsenoside Rg1（r=0.999 8）,respectively;RSD was 1.8%，1.5%，1.4% and 1.8%, respectively.Conclusion The method is simple and can be used as a quality control method for Shuxiong tinypill.