HPLC法测定草乌甲素滴丸的含量

目的:建立草乌甲素滴丸的含量测定方法。方法:采用高效液相色谱法,色谱柱为Hypersil ODS(250mm×4.0mm,5μm),流动相为乙腈-0.2%三乙胺(40:60),柱温为30℃,流速1.0mL·min-1,检测波长为261nm;对3批草乌甲素滴丸进行含量测定。结果:草乌甲素浓度在10～120μg·mL-1范围内,峰面积与浓度线性关系良好,平均回收率分别为100.33%(n=9),RSD为0.71%,3批样品中草乌甲素的含量分别为标示量的99.71%、99.78%、101.23%。结论:本法测定草乌甲素的含量,简便快捷、结果准确、重现性好、易于操作,可用于草乌甲素滴丸中草乌甲素含量的测定。

Determination of bulleyaconitine A in bulleyaconitine A dropping pills by HPLC

Objective：To establish HPLC method for determination of bulleyaconitine A in bulleyaeonitine A dropping pills. Methods： HPLC method was performed on Hypersil ODS （250mmx4.0mm,SpLm）, with acetonitrile-0.2% triethylamine solution （40：60）as mobile phase, the column temperature was 30℃, the flow rate was 1.0mL·min-1, and the UV detection wavelength was set at 261 nm. 3 batch of samples were detected. Resuhs：bulleyaeonitine A content has a linear range of 10-120μg·mL-1 with a mean recovery of 100.33%（n=9, RSD=0.71% ）. Conclusions：The method appeared to be simple, rapid, accurate and repeatable and easy to operate, it could be used for determination of bulleyaeonitine A in bulleyaconitine A dropping pills.