应用半导体激光诱导鼠脉络膜新生血管模型

目的 评价半导体激光诱导C57BL-6J小鼠脉络膜新生血管（choroidal neovascularization,CNV)模型的可行性。方法 对16只小鼠（31只眼）分别于激光照射后2h,3d,1,2及4周行荧光素眼底血管造影(fundus fluorescence angiography,FFA),光镜及电镜检查,以观察激光诱导CNV的效果。结果 激光照射后2h及3d无CNV生成,FFA显示无荧光渗漏,激光照射后1周出现CNV,FFA示1周时荧光渗漏48个点（53.3%),4周时荧光渗漏33个点（36.7%),且CNV出现不一军伴有荧光渗漏,病理切片证实,光凝后1,2及4周,小鼠CNV发生率分别为63.6%,63.6%及66.7%。结论 通过半导体激光光凝诱导C57BL-6J小鼠CNV模型是可行,有效的。

A new model of choroidal neovascularization in mice

Objective To investigate the feasibility of inducing choroidal neovascularization(CNV) in mice by diode laser Methods Two hours,3 days, 1 week, 2 weeks and 4 weeks after photocoagulation, the effect of diode laser for inducing CNV by fundus fluorescein argiography(FFA), light microscopy and transmission electron microscopy Results Two hours and 3 days after laser treatment,there were no CNV and fluorescent leakage observed in mice The first signs of leakage in FFA associated with CNV appeared 1 week after the postphotocoagulation, (48/90 lesions, or 53 3%) Four weeks after laser treatment,there were still 33 lesions appearing leakage (36 67%) Histological sections proved the existance of CNV, but the rate of CNV was not in accord with that of fluorescence leakage Conclusions This animal model is very useful for in vivo studies of angiogenesis and its modulation via various therapy