白细胞介素2在铁粉诱发早期眼内非感染性炎性反应中对晶状体上皮细胞凋亡的影响

目的研究白细胞介素2（IL-2）在眼内非感染性早期炎性反应中对晶状体上皮细胞凋亡的影响。方法将新西兰雄性大白兔随机分为对照组、实验组（晶状体内铁粉异物组）和实验对照组（晶状体内铁粉异物＋前房注射水溶性IL-2受体组）,每组8只眼。分别于手术后3、5、7、10d对各组兔眼的前房炎性反应进行观察,测定各组前房水中IL-2的水平,并对3组家兔眼做组织切片,进行信息转导与转录激活因子（STAT）1、STAT3染色及TUNEL染色。结果与实验对照组相比,实验组术后眼前房炎性反应明显,前房水中IL-2的水平明显升高。实验组STAT3染色表现为强阳性,TUNEL染色表现为弱阳性。而实验对照组STAT3染色表现为弱阳性,TUNEL染色表现为强阳性。对照组两种染色均为阴性。对照组、实验对照组和实验组晶状体上皮细胞中的STAT1染色均为阴性。结论在眼内非感染性炎性反应早期晶状体上皮细胞就可以发生凋亡。IL-2作为一种急性炎性反应因子通过激活STAT3,保护晶状体上皮细胞免于凋亡,减轻组织的炎性反应损伤。

Role of interleukin 2 on apoptosis of lens epithelium cells in intraocular non-infective inflammation induced by iron

OBJECTIVE: To observe the role of Interleukin 2 (IL-2) on the apoptosis of lens epithelium cells during the intraocular non-infective inflammation induced by iron. METHODS: Rabbits were divided into 3 groups randomly: the experimental group (injection of iron powder into the lens), the experimental control group [injection of iron and soluble IL-2 receptors (SIL-2R)] and the control group. The inflammation reaction in the anterior chamber was observed under the slitlamp biomicroscope and the level of IL-2 in the aqueous humor was assayed 3, 5, 7 and 10 days after the injection. STAT1, STAT3 and TUNEL staining were carried out on the histologic slices of enucleated eyeballs. RESULTS: The inflammation reaction in the anterior chamber in the experimental control group was less severe than that of the experimental group. IL-2 concentration in the experimental control group was lower than that of the experimental group. The STAT3 staining was strongly positive and TUNEL staining was weakly positive in the eyes of the experiment group. The STAT3 staining was weakly positive and TUNEL staining was strongly positive in the experimental control group. Both staining were negative in the control group. The STAT1 staining was negative in all 3 groups. CONCLUSIONS: Apoptosis of lens epithelium cells appears early in the intraocular inflammation induced by iron. IL-2 activates the STAT3 pathway, protects the lens epithelium cells and reduce the damage caused by inflammation reactions.