| 运用saRNA激活p21基因表达上调对肺癌细胞生长及化疗敏感性的影响 |
| |
| 引用本文： | 李占亭,师建国,卫军霞,孙脊峰,张惠中,赵洁.运用saRNA激活p21基因表达上调对肺癌细胞生长及化疗敏感性的影响[J].陕西肿瘤医学,2012(1):14-18. |
| |
| 作者姓名： | 李占亭 师建国 卫军霞 孙脊峰 张惠中 赵洁 |
| |
| 作者单位： | [1]第四军医大学唐都医院肾脏内科,陕西西安710038 [2]第四军医大学病理学系,陕西西安710032 [3]第四军医大学唐都医院中心实验室,陕西西安710038 |
| |
| 摘 要： | 目的：探讨利用针对细胞周期蛋白依赖性激酶抑制蛋白p21WAF1/CIP1（p21）的基因启动子的saR-NA（dsp21-322）特异激活p21基因的表达对肺癌细胞生长及耐药的影响。方法：化学合成针对p21启动子区的saRNA,将其转染非小细胞型肺癌细胞系A549,利用RT-PCR技术及Western blotting技术检测细胞中p21表达情况,进而观察p21表达改变后细胞凋亡情况及对顺铂药物敏感性的影响。结果：在转染dsp21-322的A549肺癌细胞中,p21的mRNA和蛋白的表达均有所上调。p21表达的上调可引起A549细胞生长的的明显抑制并可增强其对顺铂的化疗敏感性。结论：p21基因在肺癌药物耐药性的过程中发挥了作用,为应用saRNA上调抑癌基因治疗肿瘤提供了新的思路。
|
| 关 键 词： | 细胞周期蛋白依赖性激酶抑制蛋白p21 耐药性 肺癌 |
Effect of activation of p21 induced by targeted saRNA on proliferation and chemosensitivity to cispaltin in lung cancer cells |
| |
| LI Zhanting,SHI Jianguo,WEI Junxia,SUN Jifeng,ZHANG Huizhong,ZHAO Jie.Effect of activation of p21 induced by targeted saRNA on proliferation and chemosensitivity to cispaltin in lung cancer cells[J].Shaanxi Oncology Medicine,2012(1):14-18. |
| |
| Authors: | LI Zhanting SHI Jianguo WEI Junxia SUN Jifeng ZHANG Huizhong ZHAO Jie |
| |
| Institution: | 1Department of Nephrology,Tangdu Hospital,the Fourth Military Medical University,Shaanxi Xi＇an 710038,China;2Department of Pathonology,the Fourth Military Medical University,Shaanxi Xi＇an 710032,China;3Clinic Research Centre,Tangdu Hospital,the Fourth Military Medical University,Shaanxi Xi＇an 710038,China. |
| |
| Abstract: | Objective：To study the effect of up-regulating expression p21 induced by saRNA（dsp21-322） on proliferation and chemosensitivity to cispaltin in lung cancer cells.Methods： To explore the effect of up-regulation of p21 gene expression on cell proliferation and drug-resistance in A549 non-small-cell lung carcinoma cells by transfecting the dsRNA targeting the promoter region of p21 to A549 cells.Results： The enhanced p21 expression was correlated with a significant growth inhibition and enhancement of chemosensivity to cisplatin in A549 cells in vitro.In vivo experiment showed that saRNA significantly inhibited growth of xenograft tumor.Conclusion： p21 plays a role in lung cancer drug-resistance process.In addition,this study also provided evidence for the usage of saRNA as a therapeutic option for up-regulating lower-expression genes in lung cancer. |
| |
| Keywords: | cyclin-dependent kinase inhibitor p21 drug resistance lung cancer |
| 本文献已被 维普 等数据库收录！ |
|
