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| 复方莪术对MGC80—3胃癌细胞的影响 | |
| 引用本文： | 蔡祖勋,曹思之. 复方莪术对MGC80—3胃癌细胞的影响[J]. 武警医学院学报, 1996, 0(1) |
| 作者姓名： | 蔡祖勋 曹思之 |
| 作者单位： | 武警医学院外科教研室，西安医科大学—附院普外科 天津 300162，西安 710061 |
| 摘 要： | 采用MTT比色分析,~3H—TdR掺入及电镜观察复方莪术对MGC80—3胃癌细胞的影响。结果发现复方莪术能明显杀伤人低分化胃腺癌MGC80—3细胞,较低浓度即呈现明显细胞毒效应,IC_(50)为1∶150稀释度。~3H—TdR掺入试验表明:复方莪术能明显抑制MGC80—3胃癌细胞DNA的合成,掺入抑制率为78.5%。透射电镜下发现实验组细胞膜破裂,胞浆消失,呈裸核。 |
| 关 键 词： | 复方莪术 胃癌细胞 MTT比色分析 ~3H—TdR掺入 |
EFFECT OF COMPOUiND ZEDOARY ON EXTRACORPORED STOMACH CANCER CELLS MGC80-3 |
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| Cai Zuxun Cao Si/hi. EFFECT OF COMPOUiND ZEDOARY ON EXTRACORPORED STOMACH CANCER CELLS MGC80-3[J]. Acta Academiae Medicinae CPAPF, 1996, 0(1) | |
| Authors: | Cai Zuxun Cao Si/hi |
| Abstract: | This article used MTT colorimetric assay,3H -TdR incorporation assay and electronmicroscopy to observe effect of compound zedoary on extracorpored stomach cancer cells MGC80-3.The result showed that compound zedoary killed obviously human poor differentiation adenocarcinoma of cytotoxicity and its IC50 was 1:150 diluent. It had been observed in the incorporation of 3H-TdR assay that compound zedoary could inhibite significantly the DNA synthesis of MGC80-3 cell, which its inhibition rate was 78. 5 percent. Electron microscopy observation revealed that cells of the experiment group were injured and naked karyon was found. |
| Keywords: | compound zedoary stomach cancer cell MTT colorimetric assay 3H-TdR incorporation assay |
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