结缔组织生长因子在大鼠移植肺急性排斥反应期的表达及意义

目的检测结缔组织生长因子（CTGF）在大鼠移植肺内的表达,并探讨其与移植肺纤维化关系。方法Wister大鼠15只为供体,SD大鼠15只为受体,进行原位左肺移植,1周后取其中10例移植肺标本作为实验组;10例健康Wister大鼠肺标本作为对照组,采用免疫组化方法检测CTGF在肺组织中的表达,同时采用HE及Van Gieson染色观察肺组织病理形态;并进行纤维化的半定量评分。结果移植肺组织内有大量炎性细胞浸润;肺间质纤维组织增生,部分肺泡组织破坏;CTGF在移植肺组织中有明显表达,而正常肺无表达。结论在肺移植术后的急性排斥反应期CTGF有明显表达,且可能参与移植肺纤维化的病理过程。

Expression of connective tissue growth factor in acute lung allograft rejection after lung transplantation in rats

Objective To investigate the role of connective tissue growth factor (CTGF) in lung allograft rejection in rats. Methods The lungs of 15 Wister rats were transplanted into 15 Sprague-Dawely (SD) rats. The 10 transplanted lung were harvested one weeks postoperation (test group), and another 10 normal Wister rats lungs as control group. The paraffin sections of harvested lung specimens were stained by hematoxylin and eosin (HE), Van Gieson (VG) for examination of tissue morphology under microscope . The expression of CTGF was studied by immunnohistochemical method. Results There are significant inflammatory cellular infiltration in the lung tissue. The lung interstitial is light fibrosis and the alveolus is destroyed partly examined from tissue morphology in transplanted lungs. The CTGF protein was not expressed in normal rats. However, they were detected in pulmonary allograft one week postoperation. Conclusion There were expression of CTGF protein in acute pulmonary allograft rejection and those are correlated with transplanted pulmonary fibrosis.