杜仲叶水提取物对糖尿病大鼠糖脂代谢及氧化应激的影响

目的 探讨杜仲叶水提取物对糖尿病大鼠血糖、血脂及氧化应激状态的影响。 方法 采用高脂饲料喂养4周联合链脲佐菌素腹腔注射的方法复制糖尿病大鼠模型,随机分为正常对照组、模型对照组、药物对照组、杜仲叶水提取物低、高剂量(2.5、5.0g/kg)组。灌胃给药,每日1次,连续30天。检测大鼠空腹血糖(FBG)、血脂(TG、TC、LDL-C、HDL-C)、血清超氧化物歧化酶(SOD)、血清丙二醛(MDA)含量,进行统计学分析。 结果 与正常对照组比较,模型对照组大鼠FBG、TG、TC、LDL-C、MDA水平升高,HDL-C、SOD水平降低(P<0.01),提示模型复制成功。与模型对照组比较,杜仲叶水提取物(2.5、5.0g/kg)组FBG、TG、TC、LDL-C、MDA水平降低,HDL-C、SOD水平升高(P<0.01或P<0.05)。 结论 杜仲叶水提取物对糖尿病大鼠血糖、血脂有一定调节作用,推测降低氧化应激水平是其机制之一。

Effect of Eucommia Leaf Water Extract on Diabetes Rats' Lipid Metabolism and Oxidative Stress

Objective To explore the effect of Eucommia leaf water extract on diabetes rats' blood sugar, blood lipids and oxidative stress. Methods The rats have been fed with high-fat diet and have been conducted with combined intraperitoneal injection of streptozotocin for four weeks to copy diabetic rat model. They were randomly divided into normal control group, model control group, drug group, Eucommia leaf water extract low and high dose (2.5, 5.0g/kg) group. The rats were given medicine in gavage once daily for 30 consecutive days. The experimenter observes and detects the contents of fasting glucose (FBG), lipid (TG, TC, LDL-C, HDL-C), superoxide dismutase (SOD), malondialdehyde (MDA) of diabetes rats for statistical analysis. Results Compared with normal control group, model control group rats' FBG, TG, TC, LDL-C, and MDA have risen, and HDL-C as well as SOD has decreased (P<0.01), suggesting that the model has been successfully copied. Compared with the model group, Eucommia leaf extract (2.5, 5.0g/kg) group's FBG, TG, TC, LDL-C, MDA have decreased, and HDL-C as well as SOD has risen (P<0.01 or P<0.05). Conclusion Eucommia leaf water extract plays a regulatory role on the blood glucose, blood lipids of the diabetes rats. The experimenter speculates that lowering oxidative stress is part of the mechanism of the Eucommia leaf water extract.