针刺对脑卒中后抑郁大鼠行为学表现的影响

目的通过观察脑卒中后抑郁(PSD)大鼠接受不同针刺刺激后行为学表现的变化,探讨针刺治疗PSD的有效性。方法 110只雄性Sprague-Dawley大鼠应用线栓法制备脑缺血模型,将符合神经病学分级Ⅱ^Ⅳ级的60只脑缺血模型大鼠随机分为脑卒中组、PSD组、电针组、久留针组和米氮平组,每组12只;除脑卒中组大鼠正常饲养外,其余4组大鼠采用孤养与间断束缚的方法制备PSD模型,另设假手术组12只大鼠进行对照;电针组大鼠给予电针"百会"、"印堂"、左侧"内关"和"三阴交"穴,每次10 min,每日1次;久留针组大鼠给予针刺"百会"、"印堂"穴,每次120 min,每日1次;米氮平组大鼠给予米氮平片2.7 mg·kg-1,灌胃,每日1次;治疗时间均为28 d。从治疗第1天起每隔1周进行体质量、行为学方面的评估,观察针刺对PSD大鼠体质量、糖水消耗量、旷野试验方面的影响。结果各组大鼠体质量变化及糖水消耗量变化比较差异均无统计学意义(P>0.05)。治疗第1天脑卒中组、PSD组、电针组、久留针组和米氮平组大鼠水平运动评分显著低于假手术组,差异均有统计学意义(P<0.01);第8、15、22、29天PSD组大鼠水平运动评分显著低于其他各组,差异均有统计学意义(P<0.01);第15、22天久留针组大鼠水平运动评分显著高于米氮平组(P<0.05),电针组与久留针组大鼠水平运动评分比较差异无统计学意义(P>0.05);第29天,久留针组大鼠水平运动评分显著高于电针组(P<0.05),久留针组与米氮平组大鼠水平运动评分比较差异无统计学意义(P>0.05)。第1天时6组大鼠垂直运动评分比较差异均无统计学意义(P>0.05)。第8、15、22、29天,PSD组大鼠垂直运动评分逐渐减少,与其余各组比较差异均有统计学意义(P<0.01);第8、15、22、29天电针组和久留针组大鼠垂直运动评分与假手术组、脑卒中组、米氮平组比较均无统计学意义(P>0.05);电针组和久留针组大鼠垂直运动评分比较亦无统计学意义(P>0.05)。结论针刺治疗能明显改善PSD大鼠的行为学症状,其疗效与米氮平相当。

Effect of acupuncture on behavioral manifestations of rats with post-stroke depression

Objective To study the effectivity of acupuncture on post-stroke depression（PSD） in rats by observing the changes of behavioral manifestations of rats after deferent acupuncture.Methods Focal cerebral ischemia models in 110 Sprague-Dawley rats were established by line plug.Sixty cerebral ischemia rats of neurological grade Ⅱ-Ⅳ were randomly divided into cerebral apoplexy group,PSD group,electric acupuncture（EA） group,needling-retaining group and mirtazapine group,with 12 rats in each group.The rats in cerebral apoplexy group were fed normally.The PSD rats in the other four groups were established by isolated feeding and discontinuous restraint.Twelve rats were selected as sham operation group.The rats in EA group were treated with acupuncture at ＂ Baihui＂,＂ Yintang＂,left ＂ Neiguan＂ and ＂ Sanyinjiao＂; 10 minutes each time,once a day.The rats in EA group were treated with acupuncture at ＂ Baihui＂ and ＂ Yintang＂,120 minutes each time,once a day.The rats in mirtazapine group were treated with mirtazapine tablet 2.7 mg·kg^-1,once a day.The time of therapy in all rats was 28days.The body weight and behavioral manifestations of rats with PSD were evaluated every one week from the first day of treatment.The effect of acupuncture on the weight,sugar water consumption and the wilderness test of PSD rats were observed.Results There was no significant difference in the body weight and change of sugar water consumption of rats among the six groups（P〈 0.05）.The horizontal movement behavior score of rats in cerebral apoplexy group,PSD group,EA group,needlingretaining group and mirtazapine group was significantly lower than that in sham operation group on the first day of treatment（P〈 0.01）.The horizontal movement behavior score of rats in PSD group was significantly lower than that in the other groups on the 8th,15th,22nd,29th day of treatment（P〈 0.01）.On the 15th and 22nd day,the horizontal movement behavior score of rats in needling-retaining group was significantly highe