紫菀药材的高效液相色谱指纹图谱与定量分析

目的:建立紫菀药材的反相液相色谱定性和定量鉴别分析方法.方法:Ultrasphere ODS C18色谱柱(250mm ×4.6mm,5μm),流动相为甲醇-乙腈(1:2)-0.4%磷酸梯度洗脱,流速为1.0 mL/min,检测波长为360 nm,柱温为25℃.紫菀药材经80%甲醇超声处理,进行指纹图谱分析,并对紫菀药材中的主要成分槲皮素进行含量测定.结果:在该色谱条件下,6份不同紫菀药材的RP-HPLC指纹图谱中可检出10个共有峰作为定性鉴别;紫菀药材中槲皮素的含量在0.135%～0.246%之间.结论:本方法样品处理简便,准确性好,可用于紫菀药材的质量控制.

Studies on the Fingerprint and Quantitative Analyzes of Quercetin from Aster tataricus

OBJECTIVE: To establish a qualitative and quantitative method with RP-HPLC for controlling the quality of Aster tataricus L. f. METHODS: The experimental conditions of the RP-HPLC method were as follows: Ultrasphere ODS C18 columns (250 mm x 4.6 mm, 5 microm), and the mobile phase methanol-acetonitrile (1:2) -0.4% phosphoric acid, the flow rate was 1.0 ml/min, the detection wave-lengths was at 360 nm and the column temperature was 25 degrees C. The qualitative fingerprint of the samples dealt by ultrasonic bath and the quantitative measurement of quercetin were finished under the selected chromatographic conditions. RESULTS: Under the qualitative conditions, the 10 mutual peaks in RP-HPLC fingerprint of the samples from 6 different Aster tataricus L. f. can be used as index peaks for qualitative identification. In RP-HPLC quantitative analysis, the contents of quercetin in Aster tataricus L. f. were 0.135% approximately 0.246%. CONCLUSION: The analytical method is simple, quick and accurate for controlling the quality of the medicinal material of Aster tataricus L. f.