Effects of anti-phospholipid antibodies on a human trophoblast cell line (HTR-8/SVneo) |
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Authors: | Jovanović Milica Bozić Milica Kovacević Tamara Radojcić Ljiljana Petronijević Milos Vićovac Ljiljana |
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Affiliation: | a Institute for Application of Nuclear Energy - INEP, University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia b Military Medical Academy, Belgrade, Serbia c Medical Faculty, University of Belgrade, Belgrade, Serbia |
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Abstract: | Antibodies to phospholipids (aPL) have been shown to adversely affect trophoblast invasion in vivo and in vitro. HTR-8/SVneo cells derived from first trimester of pregnancy extravillous trophoblast were studied. Matrigel invasion assay, cytochemistry and cell-based enzyme-linked immunosorbant assay (ELISA) with aPL or normal IgG was used. Our data show that aPL at 100 microg/ml decrease invasiveness of HTR-8/SVneo cells to 60% of control (p<0.01), and this was also shown for primary cytotrophoblast (to 15.5% of control, p<0.001). aPL treatment caused a significant decrease in integrin alpha(1), alpha(5), and beta(1) proteins (86%, 84%, and 87%, respectively). We conclude that HTR-8/SVneo cell culture is a suitable model to study mechanisms of action of aPL on trophoblast, which in HTR-8/SVneo cells inhibit invasion by decreasing integrins alpha(5), alpha(1), and beta(1). |
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Keywords: | Extravillous trophoblast HTR-8/SVneo Anti-phospholipid syndrome Integrins Human |
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