Construction and characterization of a chimeric virus (BIV/HIV-1) carrying the bovine immunodeficiency virus gag-pol gene. |
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| Authors: | Guomin Chen Shuhui Wang Kun Xiong Jinzhong Wang Tao Ye Wenping Dong Qi Wang Qimin Chen Yunqi Geng Charles Wood Yi Zeng |
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| Affiliation: | Department of Tumor Virus and HIV, Institute of Virology, CAPM, Beijing, China. |
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| Abstract: | ![]()
HIV-1(HXB2) 5'LTR region, most of BIV(R29) gag-pol segment and HIV-1(HXB2) pol IN-3'LTR region were respectively amplified. A chimeric clone, designated as pHBIV(3753), was constructed by cloning three fragments sequentially into pUC18. MT4 cells were transfected with pHBIV3753. The replication and expressions of the chimeric virus (HBIV3753) were monitored by RT activity and IFA. The results firstly demonstrated that it is possible to generate a new type of the BIV/HIV-1 chimeric virus containing BIV gag-pol gene. |
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