用聚合酶链反应技术制备地高辛素标记核酸探针检测国人喉癌人类乳头瘤病毒DNA

应用聚合酶链反应（PCR）技术制备非放射性探针标记物——地高辛素标记人类乳头瘤病毒（HPV）共有引物探针，对146例喉不同病变的新鲜组织标本（喉癌68例，喉其它病变48例，正常喉组织30例）进行HPV6，11，16，18，31，33，35，42，58共9型HPVSDNAS感染的检测。结果表明，喉癌组HPV感染阳性率45．6％（31／68），喉癌颈转移淋巴结组阳性率21．0％（3／15），喉癌前病变阳性率11．8％（2／17），声带息肉组阳性率6．3％（1／16），15例癌旁及15例癌周正常喉组织均为HPVDNA阴性。文中还对HPV在喉癌中的致病作用及应用PCR技术制备地高李标记HPV共有引物探针的敏感性及特异性进行了讨论。

Detection of human papillomavirus in Chinese laryngeal carcinoma with digoxigenin labelled probe prepared by PCR

The studies described here detect HPV DNA in 124 cases of fresh tissue samples with different lesions of larynx using a nonradioactive Ingoxigenin Labelled Probe prepared by polymerase chain reaction (PCR). The labelled probe of HPV consensus primers was obtained,which can detect nine types of HPVs DNAs such as HPV 6, 11, 16, 18, 31, 33, 35, 42 and 58. The results showted that the rate of positive cases with HPV infection was 45. 6 % (31/68) in the group of laryngeal carcinoma, 20.0% (3/15) for the group of neck metastatic lymphnode, 11. 8% (2/17) for precarcinous lesions group and 6. 3 % (1/16) for the group of vocal cord polypus, respectively.Fifteen cases of normal laryngeal tissue adjacent to the carcinoma and 15 cases adjacent to the carcinoma were HPV DNA negative. The carcinogenic action of HPV in laryngeal carcinoma and the sensitivity and specificity of the probe prepared by PCR are discussed in this paper.