Sema3A及其受体在子宫内膜异位症中的表达及意义

目的 检测Sema 3A及其受体在子宫内膜异位症中的表达，探讨Sema 3A及其受体在子宫内膜异位症的发生和发展中的意义。方法 收集2013年1月1日至12月31日在中山大学附属第一医院妇科住院并行手术治疗的腹膜子宫内膜异位症（PEM）患者24例、卵巢子宫内膜异位囊肿（OEM）患者24例和结直肠子宫内膜异位症（CREM）患者20例，采用免疫组化SP法检测异位病灶腺上皮细胞中Sema 3A、Plexin A1和NRP-1的表达，并与26例非子宫内膜异位症患者在位内膜（EuE-NEM）、22例子宫内膜异位症患者在位内膜（EuE-EM）中的表达进行比较。结果 Sema 3A在EuE-EM组腺上皮细胞中的表达比EuE-NEM组腺上皮细胞中的表达高（309.09±66.61、207.69±56.02），差异有统计学意义（P<0.01）。PEM、OEM、CREM的异位病灶腺上皮细胞之间的Sema 3A、Plexin A1、NRP-1的免疫组织化学HSCORE评分差异均无统计学意义（P>0.05）。PEM组、OEM组、CREM组中异位病灶腺上皮细胞中的Sema 3A、Plexin A1、NRP-1的表达均比内膜异位症患者组的在位内膜和非内膜异位症患者组的在位内膜腺上皮细胞中的表达高（P值均<0.05）。结论 Sema 3A及其受体在不同类型内膜异位症病灶腺上皮细胞中的表达增高，提示其高表达可能参与了子宫内膜异位症的发生发展。

The expression and significance of Sema 3A and its receptors in endometriosis.

Abstract： Objective To study the expression of Sema 3A and its receptors (Plexin A1 and NRP-1) in endometriosis, and to explore the role of Sema 3A in the pathogenesis of endometriosis. Methods Samples were collected from 90 women undergoing endometrial biopsy or surgery for endometriosis and 26 controls receiving surgery for myoma or infertility in Department of Obstetrics and Gynecology, First Affiliated Hospital of Sun Yat-sen University (from January 1 to December 31, 2013). Immunohistochemistry was used to detect the expression of Sema 3A, Plexin A1 and NRP-1 in eutopic endometrium from women with (n=22) or without endometriosis (n=26), and in ectopic endometriotic foci of peritoneal (PEM, n=24), ovarian (OEM, n=24) and colorectal (CREM, n=20) endometriosis. The expression of three proteins were compared with those of 22 eutopic endometrium in patients with endometriosis (EuE-EM) and 26 eutopic endometrium in patients with non-endometriosis (EuE-NEM). Results The expression of Sema 3A in EuE-EM was significantly higher than that in EuE-NEM (P<0.01). There were no significant differences between the expression of Sema 3A, Plexin A1 and NRP-1 in ectopic endometrial epithelial cells of PEM, OEM and CREM (P=0.22、P=0.21、P=0.09). The expression of Sema 3A, Plexin A1 and NRP-1 in ectopic endometrial epithelial cells of peritoneal, ovarian and colorectal endometriosis was significantly higher than that of eutopic endometrial epithelial cells from patients with or without endometriosis (all of the p values were less than 0.05). Conclusion Overexpression of Sema 3A, Plexin A1 and NRP-1 in different types of endometriotic foci indicates that Sema 3A and its receptors may play an important role in the pathogenesis of endometriosis.