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| 氯化镉致人肝癌细胞SMMC-7721线粒体损伤作用 | |
| 引用本文： | 刘晓梅,石磊,王雯,金明华,杜海英,刘颖,孙磊,孙志伟. 氯化镉致人肝癌细胞SMMC-7721线粒体损伤作用[J]. 癌变．畸变．突变, 2010, 22(4): 276-278 |
| 作者姓名： | 刘晓梅 石磊 王雯 金明华 杜海英 刘颖 孙磊 孙志伟 |
| 作者单位： | 1. 吉林大学公共卫生学院,吉林,长春,130021 2. 吉林大学公共卫生学院,吉林,长春,130021;首都医科大学公共卫生与家庭医学学院,北京,100069 |
| 基金项目： | 国家自然基金青年基金 |
| 摘 要： | 目的：研究氯化镉对人肝癌细胞SMMC-7721线粒体的损伤作用。方法：分别用5、10、20、30、40μmol/L氯化镉作用体外培养的SMMC-7721细胞24h,实验同时设对照组。采用形态学方法检测其线粒体的结构完整性,用分光光度法检测线粒体钙离子转运能力,采用琼脂糖凝胶电泳法检测线粒体DNA损伤。结果：随着氯化镉的浓度升高,存活细胞数降低,细胞内的线粒体数量减少;氯化镉处理组细胞线粒体钙离子转运能力下降,并可见线粒体DNA损伤条带。结论：氯化镉可以引起人肝癌细胞SMMC-7721线粒体及线粒体DNA损伤。 |
| 关 键 词： | 氯化镉 SMMC-7721 线粒体 钙离子 |
Mitochondrial damage in SMMC-7721 cells Induced by cadmium chloride |
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| LIU Xiao-mei,SHI Lei,WANG Wen,JIN Ming-hua,DU Hai-ying,LIU Ying,SUN Lei,SUN Zhi-wei. Mitochondrial damage in SMMC-7721 cells Induced by cadmium chloride[J]. Carcinogenesis,Teratogenesis and Mutagenesis, 2010, 22(4): 276-278 | |
| Authors: | LIU Xiao-mei SHI Lei WANG Wen JIN Ming-hua DU Hai-ying LIU Ying SUN Lei SUN Zhi-wei |
| Affiliation: | 1.School of Public Health,Jilin University,Changchun 130021;2.School of Public Health and Family Medicine,Capital Medical University,Beijing 100069,China) |
| Abstract: | OBJECTIVE：The mitochondrial damage of SMMC-7721 cells induced by cadmium chloride was studied in vitro.METHODS：The SMMC-7721 cells were treated with cadmium chloride for 24 hours.Morphology was used to assess integrity of mitochondria.Spectrophotography was used to measure the transport ability of Ca^2＋ into mitochondria and agarose gel electrophoresis was used to observe the mitochondrial DNA damage.RESULTS：The mumber of treated cell and mitochondria decreased with increasing cadmium concentration.The transport ability of Ca^2＋ into mitochondria was decreased and the mitochondrial DNA was damaged with increasing concentration.CONCLUSION：Cadmium chloride could damage the mitochondria and its DNA in SMMC-7721 cells. |
| Keywords: | SMMC-7721 |
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